High-quality reference genes for quantifying the transcriptional responses of Oryza sativa L. (ssp. indica and japonica) to abiotic stress conditions  

作　　者：MAKSUP Sarunyaporn SUPAIBULWATANA Kanyaratt SELVARAJ Gopalan 

机构地区：[1]Department of Biotechnology, Faculty of Science, Mahidol University [2]Plant Biotechnology Institute, National Research Council of Canada

出　　处：《Chinese Science Bulletin》2013年第16期1919-1930,共12页

摘　　要：Rice (Oryza sativa L.) is important to food security and is also an excellent model plant for numerous cereal crops. A functional genomics study in rice includes characterization of the expression dynamics of genes by quantitative real-time PCR (qPCR) analysis; this is a significant key for developing rice varieties that perform well in the face of adverse climate change. The qPCR analysis requires the use of appropriate reference genes in order to make any quantitative interpretations meaningful. Here, the new potential reference genes were selected from a huge public database of rice microarray experiments. The expression stability of 14 candidates and 4 conventional reference genes was validated by geNorm PLUS and NormFinder software. Seven candidates are superior to the conventionally used reference genes in qPCR and three genes can be used reliably for quantitating the expression of genes involved in abiotic stress responses. These high-quality references EP (LOC_Os05g08980), HNR (LOC_Os01g71770), and TBC (LOC_Os09g34040) worked very well in three indica genotypes and one japonica genotype. One of indica genotypes including the Jasmine rice, KDML105 developed in Thailand for which no reference genes have been reported until now.Rice （Oryza sativa L.） is important to food security and is also an excellent model plant for numerous cereal crops. A functional genomics study in rice includes characterization of the expression dynamics of genes by quantitative real-time PCR （qPCR） anal- ysis; this is a significant key for developing rice varieties that perform well in the face of adverse climate change. The qPCR analysis requires the use of appropriate reference genes in order to make any quantitative interpretations meaningful. Here, the new potential reference genes were selected from a huge public database of rice microarray experiments. The expression stability of 14 candidates and 4 conventional reference genes was validated by geNormPLvs and NormFinder software. Seven candidates are superior to the conventionally used reference genes in qPCR and three genes can be used reliably for quantitating the expres- sion of genes involved in abiotic stress responses. These high-quality references EP （LOC_Os05g08980）, HNR （LOC_Os01g71770）, and TBC （LOC Os09g34040） worked very well in three indica genotypes and one japonica genotype. One of indica genotypes including the Jasmine rice, KDML105 developed in Thailand for which no reference genes have been report- ed until now.

关 键 词：功能基因组学 胁迫反应 非生物 水稻 胁迫条件 籼稻 品质 实时定量PCR 

分 类 号：S511[农业科学—作物学]