明日叶查尔酮对糖尿病大鼠肝细胞PI3K和AktmRNA表达的影响  

Effect of ashitabe chalcones on the mRNA expression of PI3K and Akt in hepatocytes of rats with diabetes

作　　者：刘贝[1] 孙建平[2] 赵阳[1] 李蕾[3] 钟进义[1] 

机构地区：[1]青岛大学医学院营养研究所,青岛266021 [2]青岛市疾病预防控制中心 [3]厦门大学公共卫生学院

出　　处：《卫生研究》2013年第3期466-469,共4页Journal of Hygiene Research

基　　金：山东省医药卫生科技发展计划项目(No.QNW001)

摘　　要：目的研究明日叶查尔酮(ashitabe chalcone,AC)对糖尿病大鼠肝细胞磷脂酰肌醇3激酶(phosphatidy I inositol 3-kinase,PI3K)和蛋白激酶(serine-threonine kinases,Akt)mRNA表达的影响。方法高脂饲料喂养加链尿佐菌素腹腔注射诱发的2型糖尿病大鼠随机分为糖尿病对照组和高、低剂量AC组,每组10只,均喂饲高脂饲料,分别每日经口灌胃给予明日叶查尔酮0、30和10mg/kg BW。另设一个正常对照组为正常大鼠喂饲普通饲料,实验周期4周。用放射免疫分析法检测血清胰岛素水平,葡萄糖氧化酶法测血糖含量,逆转录聚合酶链式反应方法检测肝细胞PI3K和Akt mRNA表达水平,蛋白印迹法测肝细胞Akt磷酸化水平。结果高剂量AC组空腹血糖和血清胰岛素水平显著低于糖尿病对照组,而PI3K mRNA、Akt mRNA和磷酸化Akt蛋白表达水平均显著高于糖尿病对照组,差异均具有统计学意义(P<0.05)。结论明日叶查尔酮可上调糖尿病大鼠PI3K和Akt mRNA的表达水平,改善胰岛素抵抗。Objective To study the effect of chalcones extracted from Angelica Keiskei（AC） on the mRNA expression of phosphatidy I inositol 3-kinase（PI3K） and serine-threonine kinases（Akt） in hepatocytes of rats with diabetes.Methods The diabetes of rats was induced by streptozotocin with intraperitoeal injection as well as with high-fat diet feeding.The rats were randomly divided into 3 groups with 10 rats in each group,diabetic control group,high-dose AC group and low-dose AC group.All the rats were fed with high-fat diet.0,30 and 10mg/kg BW AC per day were given to high-dose AC,low-dose AC and the diabetic control groups,respectively.Another 10 normal rats fed with regular diet were used as the normal control group.After 4 weeks,serum insulin levels were evaluated by radioimmunoassay.The mRNA expression levels of PI3K and Akt were determined by reverse transcription polymerase chain reaction（RT-PCR）.The phosphorylation levels of Akt in hepatocytes were detected by western blot.Blood glucose levels were measured by glucose oxidase method.Results Compared with rats in the diabetic control gruop,the levels of blood glucose and serum insulin in rats of high-dose AC gruop were decreased and the PI3K and AKT mRNA expression levels were increased.All the difference was statistically significant（P0.05）.Conclusion AC may up-regulate the mRNA expression levels of PI3K and Akt and improve insulin resistance of rats with diabetes.

关 键 词：明日叶查尔酮 磷脂酰肌醇3激酶 蛋白激酶Akt 胰岛素抵抗 

分 类 号：R587.1[医药卫生—内分泌] R282.71[医药卫生—内科学]