丹参酮ⅡA磺酸钠对海水淹溺性肺水肿大鼠的作用及其机制  

Effect of sodium tanshinone Ⅱ A sulphonate on rats with acute pulmonary edema induced by sea water drowning and the mechanism involved

作　　者：谢晓燕[1] 王永兴[1] 敬梅[1] 江玲[1] 雷燕[1] 

机构地区：[1]解放军第四五一医院呼吸科,西安710054

出　　处：《中华航海医学与高气压医学杂志》2013年第2期86-90,共5页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

摘　　要：目的探讨丹参酮ⅡA磺酸钠（sodiumtanshinone sulphonate,STS）对海水淹溺性肺水肿（ pulmonary edema of seawater downing,PE—SWD）大鼠的作用及其机制。方法将48只大鼠用数字表法随机分为正常组（normal group，NG）、STS对照（STS）组、海水（seawater，SW）组、海水＋STS治疗（SW＋STS）组，每组各12只。应用海水吸入法建立大鼠PE—SWD模型，并检测动脉氧分压（PaO：）、肺组织形态学、肺湿／干质量比值（W／D）、肺毛细血管通透性（pulmonary microvascular permeability，PMVP）及钠钾ATP酶（Na＋-K＋ -adenosine triphosphatase，NKA）的活性、肺组织匀浆中NKAal、β1的蛋白表达及mRNA丰度。结果海水吸入导致大鼠PaO：和NKA表达及活性均下降，并使W／D（6．40±0．42）、PMVP[（65．63±7．34）μg/g]增加；SW＋STS组从海水灌注后2h开始Pa02显著高于SW组；SW＋STS组的肺损伤、W／D值（5．53±0．45）及PMVP值[（52．75±5．85）μg/g]均较SW组明显减轻或减小；SW＋STS组的NKA活性、NKAal、／31蛋白丰度和NKA／31mRNA水平均较SW组明显增高，但2组NKAalmRNA水平却无明显差异。结论STS对PE—SWD大鼠有治疗作用，此作用可能与其上调NKA的表达及活性有关。Objective To investigate the effect of sodium tanshinone IIA sulphonate （STS） on rats with pulmonary edema induced by sea water drowning （PE-SWD） and the mechanism involved. Methods Forty Spragne-Dawley rats were randomly divided into 4 groups： the normal group, the STS group, the seawater group （the SW group） , the seawater ＋STS group （the SW ＋STS group） , each consisting of 12 animals. The PE-SWD rat model was established with seawater instillation. PaO2 level, pulmonary pathology, lung wet/dry ratio, pulmonary mierovascular permeability （PMVP）, the activity of Na ＋ -K ＋ -ATPase, as well as protein abundance and mRNA were monitored respectively. Results Seawater aspiration decreased the level of PaO2 and the activity of Na＋-K＋-ATPase, and at the same time increased lung wet/dry ratio and PMVP level. Two hours after aspiration of seawater, the level of PaO2 for the SW ＋ STS group increased significantly, when compared with that of the SW group. Pulmonary lesion, W/D ratio and PMVP level for the SW ＋ STS group all alleviated, when compared with those of the SW group. Na＋-K＋-ATPase activity, Na＋-K＋-ATPase al and 131 protein abundance, and Na＋ -K＋ -ATPase 131 mRNA all significantly increased, when compared with those of the SW group. However, no significant differences in the Na ＋ -K ＋ -ATPase od mRNA level could be found between the SW ＋ STS group and the SW group. Conclusions STS could produce certain effects on pulmonary edema induced by sea water drowning, the mechanism of which might be related with the level and up-regnlation of Na ＋ - K ＋ -ATPase.

关 键 词：海水 淹溺 急性肺水肿 丹参酮ⅡA磺酸钠 钠钾ATP酶 

分 类 号：R285.5[医药卫生—中药学]