过表达Mdip1基因提高番茄抗低温诱导的氧化胁迫能力  

Overexpression of Mdip1 Gene Enhanced Tolerance of Transgenic Tomato to Chilling Mediated Oxidative Stress

作　　者：晋文娟[1] 张少杰[1] 陈双臣[1] 林晓民[1] 贺超兴[2] 

机构地区：[1]河南科技大学林学院,河南洛阳471003 [2]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《植物生理学报》2013年第5期493-500,共8页Plant Physiology Journal

基　　金：国家科技支撑计划(2011BAD12B03);国家自然科学基金(31101536);山东省科技攻关计划(2012GNC011111);河南省青年骨干教师计划(2011GGJS-075)

摘　　要：采用衣杆菌介导法获得了类萌发素蛋白（germin．1ikeprotein，Mdipl）超表达的转基因番茄植株，PCR和Southern杂交检测表明，外源基因分别以1～3个拷贝整合到番茄基因组中。Real．timePCR检测表明，在转基因个体中目的基因已经在受体基因组上完成整合，且不同株系间的表达有所差异。分析了低温下转Mdipl基因株系和野生型植株efAsA、GSH含量及H2O2累计的差异。与未转基因植株相比，Mdipl超表达植株在低温胁迫下根干重、冠干重和叶绿素含量显著高于对照，并显著提高了氧化还原信号AsA、GSH含量和AsA／DHA、GSH／GsSG以及GalLDH活性，降低了低温胁迫下叶片H2O2、MDA的积累，降低程度与表达量相关。亚细胞定位表明，H2O2的增加主要分布在质外体。表明MdipI基因通过调控氧化还原信号在番茄抗氧化胁迫响应中起重要作用。The germin-like protein gene Mdipl was introduced into tomato line Ailsa Craig via dgrobacterium- mediated method. Transgenic lines were confirmed for integration into the tomato genome using PCR, Southern blot hybridization. One to three copies of the transgene were integrated into the tomato nuclear genome. Tran- scription of Mdipl in various transgenic lines was determined using real-time PCR. Performance test of resis- tance analyses to chilling stress with Tl generation transgenic tomato lines showed that the transgenic lines ex- hibited lighter symptoms of chilling injury and kept higher values of biomass accumulation and chlorophyll content than those of non-transformed plants. The resistance levels were related to expression levels of the transgene compared with wild-type plants, and the contents of AsA and AsA/DHA in both normal and low-temperature conditions were increased, while the 1-1202 and MDA content were decreased in the transgenic plants. Mean- time, the contents of GSH and GSH/GSSG and GalLDH activity were also increased in the overexpression plants. Based on these results, it can be concluded that Mdipl may play a pivotal role in increasing tomato tol- erance against chilling stress by increasing redox level and lowering H202 and lipid peroxidation accumulation.

关 键 词：番茄 氧化还原信号 类萌发素蛋白 活性氧 低温胁迫 

分 类 号：S641.2[农业科学—蔬菜学]