妊娠中晚期大鼠耐热性碱性磷酸酶及胎盘超微结构在不同二氧化碳气腹作用时间下的变化  

Effect of Carbon Dioxide Pneumoperitoneum on Level of Heat-stable Alkaline Phosphatase and Placental Ultrastructure in Medium and Late Pregnant Rats

作　　者：张颖[1] 孙冰纯[1] 钟永红[2] 廖靖[1] 

机构地区：[1]广东医学院附属医院妇产科,湛江524001 [2]粤北人民医院妇产科,韶关512025

出　　处：《生殖与避孕》2013年第5期289-293,共5页Reproduction and Contraception

基　　金：广东省科技计划资助项目(No.2008B30301019)

摘　　要：目的:探讨不同二氧化碳(CO2)气腹作用时间对妊娠中、晚期大鼠血浆耐热性碱性磷酸酶(HSAP)含量及胎盘超微结构的影响。方法:建立妊娠中期大鼠的CO2气腹模型,以气腹作用时间随机分为气腹0 h组(对照)、1 h组和2 h组,然后每组再随机分为2个亚组,即中孕组和晚孕组。用酶联免疫吸附测定法检测大鼠血浆HSAP含量,并用透射电子显微镜观察大鼠胎盘超微结构。结果:气腹1 h组孕中、晚期血浆HSAP值与同期对照组比较,差异无统计学意义(P>0.05);气腹2 h组孕晚期血浆HSAP值低于同期对照组,差异有统计学意义(P<0.05)。对照组及气腹1 h组孕中、晚期大鼠胎盘超微结构均为正常表现,气腹2 h组孕中期大鼠胎盘内质网扩张,线粒体肿胀、嵴断裂、空泡化,核内染色质异常分布;孕晚期大鼠胎盘游离缘表面微绒毛受损、排列紊乱,局部性脱失,胞质内细胞器减少,线粒体固缩变小。结论:气腹2 h组可导致妊娠中、晚期大鼠血浆HSAP下降及胎盘超微结构有缺氧表现,可能影响胎盘功能。Objective： To study the influence of carbon dioxide（CO2） pneumoperitoneum on the level of serum heat-stable alkaline phosphatase（HSAP） and the placental ultrastructure in medium and late pregnant rats.Methods： The medium pregnant rat models were set up for CO2pneumoperitoneum experiment.All the rats were randomly divided into control group,1 h group and 2 h group according to the action time of CO2 pneumoperitoneum,and then each group was divided into 2 subgroups randomly,including medium pregnancy group and late pregnancy group.The levels of serum HSAP were examined with enzyme-linked immunosorbent assay（ELISA）,and the placenta ultrastructure was observed under transmission electron microscope.Results： The level of serum HSAP in late pregnant rats in 2 h group was lower than that in the control（P0.05）,but there was no significant difference between 1 h group and the control（P0.05）.The placental ultrastructure in both medium and late pregnant rats behaved normally in both 1 h group and the control,while those in 2 h group performed hypoxia in both medium and late pregnant rats.Conclusion： Two-hour exposure of CO2pneumoperitoneum decreased the level of serum HSAP and made the placenta hypoxia in both medium and late pregnant rats,thereby may lead to function restriction of placenta.

关 键 词：CO2气腹 妊娠中晚期 耐热性碱性磷酸酶(HSAP) 胎盘超微结构 

分 类 号：R714[医药卫生—妇产科学]