湖羊催乳素受体基因外显子10多态性及与其母性行为性状的关联分析  

Analysis on Genetic Polymorphism of Exon 10 of PRLR Gene and Its Association with Distribution Type of Its Maternal Behavior in Hu Sheep

作　　者：孙伟[1] 张向楠[1] 左其生[1] 倪蓉[1] 王兰萍[2] 张有法[3] 陈玲[3] 吴文忠[3] 周洪[4] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]盐城师范学院,盐城224002 [3]苏州市畜牧兽医站,苏州215200 [4]徐州市睢宁县林牧渔业局,睢宁221200

出　　处：《畜牧兽医学报》2013年第5期673-680,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：江苏省农业科技支撑计划项目(BE2012331);科技部家养动物种质资源平台;江苏高校优势学科建设工程资助项目;江苏省研究生培养创新工程项目;国家自然科学基金(31101747);江苏省六大人才高峰项目;江苏省工程技术研究中心项目(BM2012308)

摘　　要：旨在研究催乳素受体(PRLR)基因在湖羊群体中的遗传多样性,以探讨其影响湖羊母性行为性状的可能性,为评价绵羊母性提供遗传学分析方法。以81只分娩母羊为研究对象(其中63只进行母性行为观察),采用PCR-SSCP技术检测湖羊PRLR基因外显子10基因序列的单链核苷酸多态性。运用正态分布检验、聚类分析及非参数检验法,研究湖羊母性行为分布规律和不同基因型的湖羊母性行为的差异性。结果显示,仅P3引物扩增片段存在多态性,所对应的基因型分别定义为AA、AB和BB,各基因型在群体中符合Hardy-Weinberg平衡;选取的4种母性行为:舔舐、哺乳、踩踏和拒绝哺乳的统计数据均不符合正态分布;各基因型与4种母性行为间的多个独立样本的非参数检验结果表明:舔舐、哺乳和踩踏3个母性行为在不同基因型个体间存在极显著差异(P<0.01),拒绝哺乳行为在不同基因型间则不存在显著性差异(P>0.05)。结果提示,PRLR基因外显子10部分序列存在多态性,且其与湖羊母性行为部分性状间存在一定的关联性。初步推断PRLR基因可作为湖羊母性行为的候选基因。The aim of this study was to study the genetic diversity of PRLR gene in Hu sheep to discuss its effect on Hu sheep maternal behaviors and provide the genetics analysis method for evaluating sheep maternal behaviors. 81 parturition ewes were used to detect single nucleotide polymorphism of PRLR gene exon10 by using PCR-SSCP, in which 63 individuals were observed for maternal behaviors. The methods of normal distribution test, cluster analysis and nonparametric test were used to reveal the Hu sheep maternal behavior distribution rules and the difference of Hu sheep maternal behavior with various genotypes. The result showed that only the products amplified by primers P3 displayed polymorphisms. The genotypes were AA, AB and BB, and each genotype fitted Hardy-Weinberg equilibrium. The statistical data of licking, nursing, treading and refusing to feed lamb didn’t fit normal distribution. The results of nonparametric test with multiple independent samples showed that the difference of observations for licking, nursing and trampling of individuals with AA, AB and BB genotypes was extremely significant （P〈0.01）.No obvious difference was found in individuals with three genotypes for the behaviour of refusing to feed young sheep （P〉0.05）. As the result showed, the partial sequences of PRLR gene exon10 had polymorphisms and there was correlationship between PRLR gene and maternal behaviours. According to the preliminary inference, PRLR gene could be used as candidate gene affecting the Hu sheep maternal behavior.

关 键 词：湖羊 催乳素受体基因 多态性 母性行为 

分 类 号：S826[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]