猪肺炎支原体黏附猪肾细胞模型建立及体外黏附特性研究  

Establishment of Mycoplasma hyopneumoniae Adhesion on PK15 Model and the Adhesion Ability in vitro

作　　者：张旭[1] 冯志新[1] 武昱孜[1] 车巧林[1] 刘茂军[1] 熊祺琰[1] 华利忠[1] 李彬[1] 邵国青[1] 

机构地区：[1]江苏省农业科学院兽医研究所农业部兽用生物制品工程技术重点实验室国家兽用生物制品工程技术研究中心,南京210014

出　　处：《畜牧兽医学报》2013年第5期772-777,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31001080;31001066);中国博士后基金(2012M510175)

摘　　要：为了建立猪肺炎支原体全菌体外黏附细胞模型及初步探讨其对不同细胞系的黏附特性,选取实验室保存的Mhp野毒分离株(XLW-1、XLW-2、XLW-3、WX株)、国际标准株232、弱毒疫苗168株感染猪肾传代细胞系(PK15),同时利用本实验室已建立的Mhp间接免疫荧光检测方法检测各菌株对PK15的黏附特性。结果发现,不同Mhp株全菌均可黏附PK15细胞,但不同菌株黏附能力存在差异。其黏附能力强弱依次为野毒株XLW-1株、WX株、疫苗168株、国际标准株232、野毒株XLW-2、XLW-3株。选取黏附能力较强的XLW-1株,继续探讨其对猪原代气管上皮细胞、猪肺泡上皮细胞(SJPL)、猪肺泡巨噬细胞(3D4/31)、恒河猴肾细胞(Marc145)的黏附性。XLW-1株能在体外黏附不同的细胞系,但对不同细胞的黏附能力存在差异,其中对猪源的原代分离的猪气管上皮细胞、SJPL细胞系、3D4/31细胞系黏附性均较强,而对非猪源的Marc145细胞系的黏附性较弱。本研究结果表明,Mhp全菌体可在多种细胞系上建立黏附感染模型,不同Mhp菌株的黏附能力存在着差异,且同一菌株对不同细胞系的黏附特性也存在差异。This experiment was conducted to develop the infection model of Mycoplasma hyopneumoniae in vitro and study the adhesion characteristics by indirect immunofluorescence method in different cell lines. Several Mhp strains which include field strains （XLW-1, XLW-2, XLW-3, WX）, international standard strain 232 and the attenuated strain （168） were selected, and infected Pig Kidney15 （PK15）. The results showed that all the Mhp whole bacteria strains could adhere to PK15 cell lines with different adhesion efficiency. And the order of adhesion ability of Mhp was field strain XLW-1, WX, the attenuated strain （168）, international standard strain 232, field strain XLW-2, XLW-3. The field strain XLW-1 had the best adhesion ability, so it was selected as test strain to study the adhesion ability to other cell lines, such as the original generated cells of pig tracheal epithelial, porcine alveolar epithelial cells （SJPL）, porcine alveolar macrophages （3D4/31） and rhesus monkey kidney cells （Marc145）. XLW-1 strains can adhere to different cell lines in vitro, but the different adhesion capacity also showed on the different cell lines. XLW-1 strain has good adhesion ability to pig tracheal epithelial cells in primary separation, the SJPL cell lines and 3D4/31 cell lines, while very weak ability to the non-swine cell lines, Marc145 cell line. In conclusion, Mycoplasma hyopneumoniae adhesion model was developed on different cell lines, and different Mhp strains showed different adhesion ability on the same cell lines, as well as the same strain showed the different adhesion on different cell lines.

关 键 词：猪肺炎支原体 体外感染模型 黏附特性 

分 类 号：S852.62[农业科学—基础兽医学]