地塞米松对哮喘小鼠肺组织IL-21及其受体表达的干预作用  

Effects of dexamethasone on expressions of IL-21 and its receptor in lungs of experimental asthma mice

作　　者：宫静[1] 任敦强[2] 罗雅玲[1] 赖文岩[3] 李俊红[1] 王慰盈[1] 

机构地区：[1]南方医科大学南方医院呼吸内科,广东广州510515 [2]青岛大学医学院附属医院呼吸内科,山东青岛266003 [3]南方医科大学南方医院心内科实验室,广东广州510515

出　　处：《细胞与分子免疫学杂志》2013年第6期561-564,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(30770936)

摘　　要：目的研究慢性哮喘小鼠肺组织IL-21与IL-21受体(IL-21R)表达的变化,探讨地塞米松(Dex)对小鼠肺组织IL-21与IL-21R表达的影响。方法 SPF级BALB/c雌性小鼠33只,随机分为对照组、哮喘模型组、地塞米松治疗组,每组11只。卵清白蛋白(OVA)致敏和激发建立小鼠支气管哮喘模型;HE染色观察气道炎症程度;测量支气管壁厚度及平滑肌厚度;免疫组织化学和免疫印迹法分析小鼠支气管肺组织中IL-21蛋白与IL-21R蛋白的表达。结果哮喘组小鼠支气管壁厚度及平滑肌厚度较对照组增加(P<0.01),地塞米松组支气管壁厚度与平滑肌厚度低于哮喘组(P<0.01)。哮喘组小鼠肺组织IL-21蛋白与IL-21R蛋白的表达高于对照组(P<0.05),地塞米松组IL-21蛋白与IL-21R蛋白表达低于哮喘组(P<0.05)。结论哮喘小鼠肺组织IL-21与IL-21R的表达增强,地塞米松抑制哮喘小鼠肺组织IL-21与IL-21R的表达。Objective To observe the expressions of IL-21 and its receptor （IL-21R） in the lungs of chronic asthmatic mice, and investigate the effects of dexamethasone （Dex） on their expressions. Methods Thirty-three specific pathogen free （SPF） BALB/c female mice were randomly divided into control group, asthmatic group and Dex treated group, 11 mice each group. The asthma model mice were induced by ovalbumin （OVA） with the classic method. The airway inflammation was evaluated by HE staining. The thicknesses of bronchial wall （WaVPbm） and airway smooth muscle （Wam/Pbm） were measured. The expressions of IL-21 and IL-21R proteins were detected by immunohistochemistry and Western blotting. Results Compared with the control group, the Wat/Pbm and Wam/Pbm in the asthmatic group significantly increased （ P 〈 0.01 ）. The indexes in the Dex group were significantly reduced as compared with those in the asthmatic group （P 〈 0.01 ）. The expressions of IL-21 and IL-21R proteins in the asthmatic group were higher than those in the control group （ P 〈 0.05）, but they were lower in the Dex group than in the asthmatic group （ P 〈 0.05）. Conclusion The enhanced expressions of IL-21 and IL-21R in the asthmatic mice are found, and Dex can inhibit the expressions of IL-21 and IL-2IR in the lung tissues of asthmatic mice.

关 键 词：IL-21 哮喘 地塞米松 小鼠 

分 类 号：R562.25[医药卫生—呼吸系统] R392-33[医药卫生—内科学]