血管紧张素-(1-7)抑制低氧诱导的大鼠肾小管上皮细胞转分化  

Angiotensin-(1-7) inhibits hypoxia-induced renal tubular epithelial-to-mesenchymal transition in rats

作　　者：任婷婷[1] 贺红焰[2] 喻小兰 樊均明[1] 谭剑[1] 刘建[1] 

机构地区：[1]泸州医学院附属医院肾内科,四川泸州646000 [2]泸州医学院附属医院病理科,四川泸州646000 [3]泸州医学院附属医院 [4]泸州市龙马潭妇幼保健院,四川泸州646000

出　　处：《细胞与分子免疫学杂志》2013年第6期593-596,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(30771008)

摘　　要：目的观察血管紧张素-(1-7)[Ang(1-7)]对氯化钴(Co)诱导的低氧状态下正常大鼠近端肾小管上皮细胞(NRK52E)间质转分化的影响并探讨其可能机制。方法体外培养NRK52E细胞,分为对照组、Co组、Ang-(1-7)组、Co+Ang-(1-7)组,培养6 d。免疫细胞化学染色法检测NRK52E细胞低氧诱导因子-lα(HIF-lα)和α-平滑肌肌动蛋白(α-SMA)的表达情况;Western blot法、细胞免疫化学染色法检测p-ERK1/2蛋白表达水平;酶联免疫吸附法检测细胞上清液Ⅰ型胶原蛋白(Col-Ⅰ)的含量。结果 6 d后,与对照组比较,Co组与Co+Ang-(1-7)组细胞HIF-1α、α-SMA、ColⅠ及p-ERK1/2表达量显著增加(P<0.05);与Co组比较,Co+Ang-(1-7)组细胞HIF-1α、α-SMA、ColⅠ及p-ERK1/2表达量明显减少(P<0.05)。结论 Ang-(1-7)可抑制Co诱导的低氧状态下肾小管上皮细胞间质转分化,减少细胞外基质的生成,可能是通过p-ERK1/2信号通路实现的。Objective To investigate the effect of angiotensin-（1-7） [ Ang（1-7） ] on the transdifferentiation of normal rat kidney proximal tubulαr epithelia cells （NRK52E） under hypoxic condition induced by cobaltous chloride （Co） and the under- lying mechanism. Methods NRK52E cells were divided into control group, Co group, Co ＋Ang-（1-7） group and Ang-（1-7） group and cultured for 6 d. Expression levels of hypoxiα-inducible factor-lα （HIF-lα） and α-smooth muscle actin （α-SMA） were detected by immunocytochemistry. Immunohistochemistry and Western blotting were used to determine the expression of p-ERK1/2 and ELISA to measure the content of collαgen type 1 （Col I ） in the culture supernatant. Results Compared with the control group, the expressions of HIF-lα, α-SMA, Col I and p-ERK1/2 in the Co group and the Co ＋ Ang-（1-7） group increased significantly （P 〈0.05） 6 d lαter. Compared with the Co group, the expressions of HIF-lα, α-SMA, Col l and p-ERKI/2 in the Co ＋ Ang-（1-7） group decreased significantly （ P 〈 0. 05）. Conclusion Ang-（1-7） can inhibit Co-in- duced rats＇ tubulαr epithelial-to-mesenchymal transition and reduce the production of extracellulαr matrix. Inhibition of ERK1/ 2 pathway may plαy an important role in this process.

关 键 词：上皮间质转分化 低氧诱导因子-lα 低氧 血管紧张素-(1-7) 

分 类 号：R392-33[医药卫生—免疫学] R692.6[医药卫生—基础医学]