可溶型B7-H3在原发性肝细胞癌患者血清中的表达及临床意义  

Expression and clinical significance of soluble B7-H3 in sera of patients with primary hepatocellular carcinoma

作　　者：王斐斐[1] 于国华[2] 刘同慎[1] 王国艳[1] 杨静 张光波[4] 栾希英[1] 

机构地区：[1]滨州医学院免疫教研室,山东烟台264003 [2]山东省烟台毓璜顶医院,山东烟台264003 [3]山东省烟台胜利油田疗养院,山东烟台264003 [4]苏州大学医学生物技术研究所,江苏苏州215007

出　　处：《细胞与分子免疫学杂志》2013年第6期629-632,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(30972778);山东省自然科学基金(ZR2010HL048)

摘　　要：目的检测协同刺激分子B7-H3在肝细胞癌患者血清及组织中的表达,并探讨其临床意义。方法选取原发性肝细胞癌63例患者的癌组织和血清标本;5例肝血管瘤的瘤旁组织作为正常肝组织对照;同期收取50例健康体检人员外周血标本为正常对照。采用ELISA检测肝癌患者和正常人血清中可溶型B7-H3(sB7-H3)的含量,免疫组化检测正常肝组织和肝细胞癌组织中B7-H3的表达。结果肝细胞癌患者外周血清sB7-H3含量为(4143.47±976.27)pg/mL,显著高于正常对照组(2076.18±605.42)pg/mL(P<0.05);且其表达水平与临床分期,是否远处转移及肝癌组织中是否阳性表达B7-H3等参数有关(P<0.05),与年龄、性别、组织学类型、淋巴结转移及肿瘤大小等临床病理参数无关;与血清学指标CA19-9呈明显正相关(P<0.05),但与AFP和CEA水平无相关性。结论肝癌患者血清中sB7-H3表达明显高于正常人,B7-H3表达与疾病临床病理指标相关,表明检测sB7-H3可能对原发性肝癌的诊断有一定价值。Objective To detect the expression and clinical significance of co-stimulatory molecule B7-H3 in sere and tissues of patients with hepatocellular carcinoma （HCC）. Methods We collected 63 samples of sera and liver tissue from HCC patients, 5 adjacent normal tissues of hepatic hemangioma as controls, and the other 50 sera samples of healthy people in the same time. The expression of soluble BT-H3 （sB7-H3） in sera from HCC patients and healthy people was detected by ELISA. Immunohistochemistry was performed to analyze the expression of B7-H3 on normal liver and HCC tissues. Results The level of sBT-H3 from HCC patients was significantly higher than that from healthy people [ （4143.47±976.27） pg/mL vs （2076.18 ±605.42） pg/mL, P 〈 0.05 ]; and sBT-H3 level was related to clinical stage, distant metastasis and the positive expression of B7-H3 in HCC tissues （ P 〈 0.05）, while there was no significant difference between its expression and the oth- er clinical pathological parameters, such as the patients＇ age, gender, histological type, lymphatic metastasis and size of tumor. Furthermore, a positive correlation was found between the level of sB7-H3 and CA19-0 in HCC patients （P〈0.05）, but it was not associated with AFP and CEA. Conclusion The level of sB7-H3 in HCC patients was significantly higher than that in healthy people, and BT-H3 expression was correlated with clinical pathological indexes, which implicated that detecting the expression of sB7- H3 might be helpful for the diagnosis of primary HCC.

关 键 词：共刺激分子 B7-H3 肝细胞癌 ELISA 

分 类 号：R392.11[医药卫生—免疫学] R735.7[医药卫生—基础医学]