Wnt2在乳腺癌组织及患者血清中的表达及意义  

Expression Wnt2 in breast cancer tissues and sera of the patients

作　　者：郭变琴[1] 吴立翔[2] 汪先桃[1] 涂植光[1] 

机构地区：[1]重庆医科大学临床检验诊断学教育部重点实验室,重庆400016 [2]重庆市肿瘤医院检验科,重庆400030

出　　处：《细胞与分子免疫学杂志》2013年第6期633-636,共4页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的检测乳腺癌细胞株(MDA-MB-231、ZR-75-30、MCF-7)、乳腺癌患者组织中Wnt2的表达,同时检测乳腺癌患者血清中Wnt2及CA-153表达量,并对二者的相关性进行分析。方法采用实时荧光定量PCR及Western blot技术检测MDA-MB-231、ZR-75-30、MCF-7中Wnt2 mRNA及蛋白水平;采用免疫组织化学对5例配对的癌旁组织、9例乳腺原位癌及9例侵袭性乳腺癌进行检测;同时采用ELISA检测15例健康成年女性、30例乳腺癌患者血清中Wnt2蛋白水平;电化学发光技术检测CA-153水平,并收集完整的病理资料与血清中Wnt2蛋白水平比较分析。结果 Wnt2在乳腺癌细胞株MDA-MB-231、ZR-75-30、MCF-7中未见表达;在乳腺癌组织上皮及间质细胞中呈高表达,而在配对的癌旁组织呈不表达或弱表达状态;30例乳腺癌患者血清中Wnt2及CA-153平均表达量显著高于健康成年女性(P<0.05),且Wnt2表达水平与CA-153呈正相关。结论血清Wnt2可与CA-153同时作为乳腺癌筛查的血清学辅助指标,有助于提高乳腺癌检出的灵敏度。Objective To observe the expression of Wnt2 in the breast cancer cells （ MDA-MB-231, ZR-75-30 and MCF-7） and tissues, and analyze the correlation of Wnt：2 expression with CA-153 in the sera of patients with breast cancer. Methods The expression of Wnt2 at both mRNA and protein levels was measured respectively by real-time fluorescent quantitative PCR and Western blotting in the three human breast cancer cell lines. In addition, the immunohistochemistry （IHC） was ap- plied to detect the expression of Wnt2 in 5 corresponding tissues adjacent to cancer, 9 breast carcinoma in situ and 9 invasive breast cancer; ELISA was used to detect Wnt2 and electrochemiluminescence immunoassay was used to detect CA-153 in the sera of 15 normal women and 30 patients with breast cancer. Furthermore, we collected complete clinicopathological data from the participating subjects, and analyzed their relationships with serum Wnt2 levels. Results The mRNA and protein of Wnt2 were negative in the three human breast cancer cells, while Wnt2 in the epithelial and interstitial cells of breast cancer tissues was significantly higher than that in normal tissues （ P 〈 0.05）. The expression levels of Wnt2 and CA-153 in the sera of patients with breast cancer were higher than those in normal women （ P 〈 0.0.5）, and Wnt2 levels in the sera of breast cancer patients were positively correlated with serum CA-153 levels. Conclusion Simultaneous tests of Wnt2 and CA-153 levels in the serum may help the diagnostic screening of breast cancer.

关 键 词：Wnt2 CA-153 乳腺癌 实时荧光定量PCR 蛋白印迹 免疫组织化学 酶联免疫吸附试验 

分 类 号：R392.33[医药卫生—免疫学] R737-9[医药卫生—基础医学]