灵芝多糖在Graves病小鼠模型中对甲亢及甲亢肝脏功能损伤的影响  

Effect of Ganoderma lucidum polysaccharides on hyperthyroidism and liver injury in Graves' disease mice model

作　　者：赵泽飞[1] 赵咏桔[2] 顾明君[1] 崔九兰 王曙[4] 

机构地区：[1]上海市浦东新区公利医院内分泌代谢病科,上海200135 [2]上海交通大学医学院附属瑞金医院老年病科,上海200025 [3]上海市内分泌代谢病研究所,上海200025 [4]上海交通大学医学院附属瑞金医院内分泌代谢病科,上海200025

出　　处：《上海交通大学学报（医学版）》2013年第5期607-610,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海市卫生局青年科研项目(2010Y054);上海市浦东新区卫生局卫生科研项目(青年基金)(PW2010B-3);上海市浦东新区卫生系统优秀青年医学人才项目(PWRq2010-02)~~

摘　　要：目的探讨灵芝多糖对Graves病(GD)模型小鼠甲状腺功能亢进症(甲亢)及其所致肝脏功能损伤的影响。方法利用促甲状腺素受体A亚单位(TSHR-A)免疫BALB/c小鼠获得GD动物模型,检测小鼠血清甲状腺素(T4)、促甲状腺素受体抗体(TRAb)和肝功能指标并分析相关性。在建模过程中,分别给予纯水、低剂量灵芝多糖(100 mg.kg-1.d-1)和高剂量灵芝多糖(400 mg.kg-1.d-1)灌胃,观察小鼠血清T4、TRAb和肝功能指标谷丙转氨酶(ALT)、谷草转氨酶(AST)和碱性磷酸酶(ALP)的变化。结果与模型组未发生甲亢和对照组小鼠比较,模型组发生甲亢小鼠的血清ALT、AST和ALP水平均显著升高(P<0.05),血清AST、ALP与T4水平呈显著正相关(r值分别为0.585和0.744,P<0.05),与TRAb水平无相关性(P>0.05)。灵芝多糖干预后,模型小鼠血清T4和TRAb无显著变化(P>0.05)。经低剂量灵芝多糖干预的模型组发生甲亢小鼠的肝功能指标(血清ALT和ALP)均显著优于纯水组(P<0.05)。结论灵芝多糖对GD模型小鼠的甲亢状态无明显影响,但对甲亢引起的肝脏功能损伤具有改善作用。Objective To investigate the effect of Ganoderma lucidum polysaccharides on hyperthyroidism and liver injury in the mice model of Graves＇ disease （ GD）. Methods GD mice model was constructed by immunizing BALB/c mice with thyroid stimulating hormone receptor-A （TSHR-A）. Serum thyroxine （T4）, thyroid stimulating hormone receptor antibody （TRAb）, and liver function parameters were measured, and correlation analysis was performed. The mice were intragastrically managed with pure water, low-dose Ganoderma lucidum polysaccharides （100 rag. kg -1·d-1） and high-dose Ganoderma lucidum polysaecharides （400 mg·kg-1·d-l） respectively, and the change of serum T4, TRAb, alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, and alkaline phosphatase （ALP） were determined. Results Serum ALT, AST and ALP in mice with hyperthyroidism in model group were significantly higher than those in mice without hyperthyroidism in model group and mice in control group （P 〈 0.05）. Serum AST and ALP were positively significantly related to serum T4 （ r = 0. 585, P 〈 0.05; r = 0. 744, P 〈 0.05）, but were not related to serum TRAb （P 〉 0.05）. Serum T4 and TRAb in model mice were not significantly changed after treatment with Ganoderma lucidum polysaccharides （P 〉 0.05）. Serum ALT and ALP in mice with hyperthyroidism in model group treated with low-dose Ganoderma lucidumpolysaccharides were significantly superior to those in pure water group （P 〈 O. 05）.Conclusion Ganoderma lucidumpolysaccharides may not work on hyperthyroidism improvement in GD mice model, but can improve the liver injury caused by hyperthyroidism.

关 键 词：GRAVES病 灵芝多糖 甲状腺功能亢进症 肝脏损伤 

分 类 号：R581.1[医药卫生—内分泌]