二甲双胍对2型糖尿病大鼠骨骼肌SIRT3表达的影响  

Effect of metformin on expression of SIRT3 in skeletal muscle of rats with type 2 diabetes

作　　者：宋玉萍[1] 韩冲[1] 史婧丽[1] 吴莹[1] 刘志民[1] 

机构地区：[1]第二军医大学附属长征医院内分泌科,上海200003

出　　处：《中华内分泌代谢杂志》2013年第5期427-429,共3页Chinese Journal of Endocrinology and Metabolism

基　　金：国家重点基础研究发展计划（“973”计划,2005CB523304）

摘　　要：采用高脂饮食加腹腔注射链脲佐菌素制备2型糖尿病大鼠模型，二甲双胍干预4周，实时定量PCR及Western印迹法分别检测大鼠肌肉组织SIRT3 mRNA及蛋白水平。结果显示，与对照组相比，糖尿病组大鼠胰岛素敏感性及超氧化物歧化酶（SOD）、谷胱甘肽（GSH）水平明显下降（P〈0.05），丙二醛水平明显升高（P〈0.05）, 骨骼肌SIRT3 mRNA及蛋白水平明显下降（P〈0.01）。经二甲双胍治疗后大鼠胰岛素敏感性及SOD、GSH、SIRT3 mRNA及蛋白水平明显升高（P〈0.05或P〈0.01）, 丙二醛水平下降（P〈0.05）。提示二甲双胍可能通过上调糖尿病大鼠骨骼肌SIRT3的表达增加胰岛素敏感性。Type 2 diabetic rat model accompanied by insulin resistance was established by high fat/high glucose diet and streptozotocin. Following metformin treatment for 4 weeks, realtime PCR and Western blot were used to detect the expressions of SIRT3 mRNA and protein in skeletal muscle tissue, respectively. The results showed that insulin sensitivity, superoxide dismutase （SOD）, and glutathione （GSH） levels were significantly reduced in diabetic group compared with the control group （P〈0.05）, while methane dicarboxylic aldehyde （MDA） level was increased （P〈0.05）, along with the decreased expressions of SIRT3 mRNA and protein in skeletal muscles （P〈0.01）. After metformin treatment, the insulin sensitivity, SOD, GSH, and SIRT3 mRNA and protein levels were significantly increased （P〈0.05 or P〈0.01）, while MDA level was decreased （P〈0.05）, suggesting that metformin may ameliorate insulin resistance via upregulating SIRT3 expression in skeletal muscles of diabetic rats.

关 键 词：二甲双胍 SIRT3 糖尿病 2型 大鼠 

分 类 号：R587.1[医药卫生—内分泌]