紫外分光光度计测定小儿速效感冒口服溶液中对乙酰氨基酚含量的4种定量分析方法比较  

Comparison of Content Determination of Acetaminophen in Xiaoer Suxiao Ganmao Oral Solution by 4 Quantitative Analysis Methods with UV Spectrophotometer

作　　者：景霞[1] 徐进[1] 初立梅[1] 李文静[1] 

机构地区：[1]南京医科大学附属南京儿童医院药学部,南京210008

出　　处：《中国药房》2013年第21期1996-1998,共3页China Pharmacy

摘　　要：目的:比较紫外分光光度计测定小儿速效感冒口服溶液中对乙酰氨基酚含量的4种定量分析方法。方法:采用双波长法(测定波长257.2nm、参比波长283.4nm)、一阶导数法(271nm)、单波长校正曲线法(257.2nm)、吸光系数法(257.2nm)对紫外扫描光谱数据进行定量分析,并进行4种方法的方法学考察。结果:4种方法中对乙酰氨基酚检测质量浓度线性范围均为2～10μg/ml(r≥0.9998),平均回收率为99.30%～99.84%,批内RSD分别为0.13%、0.01%、0.27%、0.27%,批间RSD分别为0.18%、0.01%、0.37%、0.37%。结论:上述4种分析方法都可用于小儿速效感冒口服溶液中对乙酰氨基酚的含量测定,但双波长法和一阶导数法因操作更简便更适合医院制剂的测定。OBJECTIVE：To compare 4 quantitative analysis methods for the determination of acetaminophen content in Xiaoer suxiao ganmao oral solution with UV spectrophotometer.METHODS：UV scanning spectrum was analyzed quantitatively by dual-wavelength spectrophotometry（detection wavelength of 257.2 nm,reference wavelength of 283.4 nm）,first-derivative spectrophotometry（271 nm）,single-wavelength calibration curve method（257.2 nm）and absorbancy index（257.2 nm）.Methodology of 4 methods were all investigated.RESULTS：The linear range of acetaminophen were 2-10 μg/ml（r≥0.999 8）by 4 methods with average recoveries of 99.30%-99.84%;RSDs were 0.13%,0.01%,0.27% and 0.27% for within-run assays,and 0.18%,0.01%,0.37% and 0.37% for between-run assays.CONCLUSIONS：Above 4 methods can be used for the determination of acetaminophen in Xiaoer suxiao ganmao oral solution,and dual-wavelength spectrophotometry and first-derivative spectrophotometry are simpler and more convenient for the determination of hospital preparation.

关 键 词：小儿速效感冒口服溶液 对乙酰氨基酚 含量 紫外光谱扫描 双波长法 一阶导数法 单波长校正曲线法 吸光系数法 

分 类 号：R927.2[医药卫生—药学] R971.1