慢性毒性联合致癌性试验中SD大鼠血液部分生理生化参考值的研究  

Reference Value for Body Weight、Mortality、Hematology and Serum Biochemistry of SD Rats in the Combined Chronic Toxicity/Carcinogenicity Study

作　　者：钟志勇[1] 刘盛来[1] 郑桂兰[1] 赵士海[1] 谢超敏[1] 王刚[1] 黄建勋[2] 唐小江[1] 

机构地区：[1]广东省医学实验动物中心,佛山528248 [2]广东省职业病防治院,广州510300

出　　处：《实验动物科学》2013年第2期14-19,共6页Laboratory Animal Science

摘　　要：目的研究两年慢性毒性联合致癌性试验中SD大鼠的体质量、死亡率、血常规值和血生化值的变化。方法SPF级雄性SD大鼠135只,雌性138只,4～17周龄间每周测量体质量1次,18～108周龄间每4周测定动物体质量1次并计算动物的死亡率;分别于8周龄、30周龄、56周龄、82周龄和108周采血,检测血常规和血生化指标。结果雌性大鼠在6～108周的体质量与雄性大鼠比较具有显著性差异(P<0.05);82～108周龄是大鼠死亡率增长的快速期。随着周龄的增加,雌鼠和雄鼠血液指标的变异系数增大。性别对大鼠的血常规和血生化指标具有明显的影响,但雌鼠与雄鼠比较具有显著性差异的血液指标,随着周龄的增加而减少。结论在进行安全性评价试验中,性别、周龄对SD大鼠体质量、血常规值、血生化指标均有较大的影响,因此,雌雄宜分开统计,并需使用相同周龄的动物背景数据作为参考值范围。Objective To investigate the change of reference value for body weight,mortality,hematology and serum biochemistry of SD rats in test method of combined chronic toxicity/carcinogenicity study. Method 135 male and 138 female specific pathogen free SD rat of 4 weeks were selected. We measured the body weights once a week between 4 - 17 weeks of age and once four week between 18 - 108 weeks of age. The indexes of hematology and biochemistry of SD rats were measured at the age of 8 weeks old,30 weeks old,56 weeks old,82 weeks old,108 weeks old and record the mortality rate every day. Result The body weight of male rats was much higher than female rats from 6 weeks old. The 82 - 108 weeks of age was the rapid growth of mortality in rats. The coefficient of variation of indexes of hematology and biochemistry of male and female become larger with increasing age. Gender has a significant impact on hematology and serum biochemistry in rats. But the hematology and serum biochemistry which has significant difference between female and male become less with increasing age. Conclusion During the safety evaluation tests,expecially combined chronic toxicity/ carcinogenicity study, both of gender and age have a greater impact on the body weight, hematology and serum biochemistry. Male and female should be separated from the statistics and the animals background data should be used at the same weeks.

关 键 词：慢性毒性联合致癌性试验 SD大鼠 体质量 死亡率 血常规值 血生化值 背景数据 

分 类 号：Q95-33[生物学—动物学]