免疫损伤结合高脂饲料致兔动脉粥样硬化斑块形成的相关因素分析  

Relative Factors for Experimental Atherosclerotic Plaque of Rabbit Induced by Immunolesion and High Fat Diet

作　　者：刘剑刚[1] 董国菊[1] 马鲁波[1] 史大卓[1] 

机构地区：[1]中国中医科学院西苑医院心血管病中心,北京100091

出　　处：《实验动物科学》2013年第2期32-38,共7页Laboratory Animal Science

基　　金：国家重点基础研究发展规划项目(No.G19990544-05)

摘　　要：目的利用免疫损伤结合高脂饲料方法建立兔实验性动脉粥样硬化(AS)斑块形成模型,探讨影响其斑块形成的相关因素。方法日本大耳白兔30只。免疫损伤和高脂饲料组兔经耳缘静脉注射牛血清白蛋白(BSA)生理盐水溶液(250mg/kg)并喂食高脂饲料,7 d后再次经耳缘静脉注射同等剂量的BSA。另设单纯喂养高脂饲料的AS模型组和普通饲料喂食的正常兔组,72 d后取血测定动物的血脂指标、炎症因子、血管活性物质、血小板聚集黏附及血管内皮功能等指标,分析上述测定指标和兔主动脉斑块形成及病理变化的相关性。结果 72 d后,免疫损伤结合高脂饲料组和单纯高脂饲料的两组经病理观察主动脉均有斑块形成,其前者的血脂水平、血清炎症因子白介素-6(IL-6)、白介素-8(IL-8)、高敏C-反应蛋白(hs-CRP)含量均比单纯高脂饲料组显著增高(P<0.05,P<0.01)。经Pearson相关系数统计处理,上述指标和免疫损伤加高脂饲料致斑块的形成具有正相关性,和HDL-C(r=-0.58)、NO(r=-0.26)等指标具有负相关性。结论免疫损伤结合高脂饲料导致兔AS斑块形成和血脂水平、炎症因子、血小板聚集及血管活性物质等经典指标的异常有显著相关性,具有评价价值。Objective To investigate the relative factors for experimental atherosclerotic plaque of rabbit induced by immunolesion and high fat diet. Method 30 Japanese white rabbits, intravenously injected bovine serum albumin （250mg/kg） , feeding high fat diet after injection. 7 days later, injected again bovine serum albumin （250mg/ kg）. Meanwhile, established purely high fat diet group and normal diet group as control, 10 rabbits each group. 72 days later, got blood from central artery of rabbit ears to determine lipid level, inflammation factors, vasoactive substance, platelet aggregation and vascular endothelium, etc. and analyzed which related to the pathological change of atherosclerotic plaque or not with SAS statistic software. Result After 72 days, the aorta of both the test group and the purely high fat diet group formed plaque. The plaque of the test group is localized, which is similar to the human being. The lipid level and the content of inflammatory factors, e. g. , interleukin - 6 （ IL - 6） , interleukin- 8 （IL- 8） and high sensitivity-C reactive protein （hs-CRP） are obviously higher than the control group ; the content of vasoactive substance and thromboxane B2 （ TXB2 ） changed significantly than the control group （P 〈 0.05）. By the Pearson correlation coefficient statistics processing, And above factor into positive correlation to the plaque size induced by immunolesion and high fat diet; high density lipoprotein （ HDL-C, r = - 0.58） ）, nitric oxide （NO,r = - 0.26） are negatively related with the plaque size, and the correlation coefficient of HDL-C is the greatest. Conclusion The rabbit atherosclerotic plaque induced by immunolesion and high fat diet formed in a short time with obvious pathological change, which is similar to that of human beings and closely related to lipid level, inflammation factor, platlet aggregation and endothelial factor, which further show it more reasonable for evaluation the atherosclerosis.

关 键 词：动脉粥样硬化 斑块形成 脂质水平 炎症因子 血小板聚集 血管活性物质 

分 类 号：S852.5[农业科学—基础兽医学] R364.5[农业科学—兽医学]