诱导痰P物质和白细胞介素-4检测在咳嗽变异性哮喘诊治中的临床价值  

Value of levels of SP and IL-4 in the induced sputum in the diagnosis and treatment of the patients with cough variant asthma

作　　者：叶飒[1] 严建平[1] 王宏[1] 

机构地区：[1]浙江省人民医院呼吸内科,浙江杭州310014

出　　处：《全科医学临床与教育》2013年第3期256-258,共3页Clinical Education of General Practice

摘　　要：目的检测咳嗽变异性哮喘(CVA)患者诱导痰中P物质(SP)和白细胞介素-4(IL-4)的表达水平,探讨SP和IL-4在CVA中的作用。方法选择30例CVA患者作为CVA组,30例健康者作为对照组。对两组受试者进行痰诱导,计数对照组及治疗前后CVA组诱导痰中嗜酸性粒细胞(EOS)的百分比;酶联免疫吸附双抗体夹心法(ELISA法)检测对照组及治疗前后CVA组诱导痰中SP和IL-4的表达水平。结果 CVA组诱导痰中EOS百分比、SP和IL-4水平均高于对照组,差异具有统计学意义(t分别=12.28、9.35、6.51,P均<0.05);CVA组治疗后诱导痰中EOS百分数、SP和IL-4水平与治疗前比较,差异具有统计学意义(t分别=7.96、6.26、5.31,P均<0.05)。诱导痰中SP,IL-4表达水平与EOS百分比呈正相关(r分别=0.55、0.60,P均<0.05),诱导痰中SP和IL-4水平呈正相关(r=0.52,P<0.05)。结论 EOS的浸润和炎性介质SP、IL-4的升高参与了CVA的发病过程,糖皮质激素联合长效β2受体激动剂吸入治疗能显著降低诱导痰中EOS、SP和IL-4水平。Objective To examine the levels of SP and IL-4 in the induced sputum of the patients with CVA, to study the roles of SP and IL-4 in CVA. Methods Thirty patients with CVA were enrolled in the study（group CVA）, as well as 30 healthy controls. The induced sputum were obtained in each group and the percentage of EOS was counted in control group and CVA group before and after treatment ; in the induced sputum＇s supernatant, the levels of the SP and IL-4 were determined by ELISA in each group. Results EOS percentage ,levels of SP and IL-4 of CVA patients were significantly higher than that of the control group （t= 12.28,9.35,6.51 ,P〈0.05 ）. EOS percentage,levels of SP and IL-4 of CVA patients after treatment had significantly differences compared with that before treatment （t=7.96,6.26,5.31,P〈0.05 ）. The levels of SP and IL-4 were positively correlated with the percentage of EOS （r=0.55,0.60,P〈0.05） ; the level of SP was positively correlated with the level of IL-4（r=0.52,P〈0.05）. Conclusions EOS infiltration and increase expression of SP and IL-4 play key roles in the pathogenesis of CVA; the joint inhaled long-acting beta agonist and glucocorticoids can significantly decrease the levels of EOS , SP and IL-4 in the induced sputum.

关 键 词：咳嗽变异性哮喘 诱导痰 嗜酸性粒细胞 P物质 白介素-4 

分 类 号：R562.25[医药卫生—呼吸系统]