斯卑尔脱小麦Vp-1B基因的等位变异鉴定和序列分析  

Identification and Analyses of Vp-1B Allelic Variation in Triticum spelta L

作　　者：曲若端[1] 杨燕[1] 孟建宇[1] 李淑芬[1] 赵宏斌[1] 

机构地区：[1]内蒙古农业大学生命科学学院,呼和浩特010018

出　　处：《内蒙古大学学报（自然科学版）》2013年第3期286-293,共8页Journal of Inner Mongolia University：Natural Science Edition

基　　金：国家自然科学基金项目(31160247;31260320);内蒙古农业大学科技创新团队基金项目(ND-PYTD2010-3);国家春晖项目计划(Z2009-1-01060)

摘　　要：斯卑尔脱小麦是小麦的一级基因源.在影响小麦穗发芽的众多基因中,Vp-1是调节胚发育,促进胚成熟和休眠的主要转录调节因子.本研究通过同源克隆测序的方法分析了4种不同基因型斯卑尔脱小麦品种Vp-1基因在3B染色体上的等位变异,进一步分析了Vp-1基因在斯卑尔脱小麦和六倍体普通小麦中的差异.研究表明不同休眠特性的4份斯卑尔脱小麦Spelta80,Spelta220,Spelta217和Spelta225在3B染色体的Vp-1B基因上存在大量的等位变异,主要集中在Vp-1B基因的第三和第五内含子中,表现为一些大片段的序列缺失,另外的变异位于Vp-1B基因的第一外显子区和第六外显子区的SNP位点,引起了氨基酸的改变.根据测得的正反向序列重叠部分判断其准确性并拼接全长,命名了斯卑尔脱小麦中存在的三种等位变异,分别为SVp-1Bc(第三内含子中缺失83bp)、SVp-1Bd(第三内含子中缺失25bp)和SVp-1Be(第五内含子中缺失10bp).从斯卑尔脱小麦与六倍体普通小麦的亲缘关系来看,斯卑尔脱小麦中存在的Vp-1B基因的等位变异极有可能也与其休眠特性相关.Triticurn spelta L is one of the first level genetic resources of wheat. Among many genes that can affect the Pre-harvest sprouting,the viviparous-1 （Vp-1） gene is the main impor- tant regulator that can promotes mature and dormancy of embryo in bread wheat. In this study, by the method of homologous cloning, the PCR fragments in Spelta 80, Spelta 225, Spelta 217 and Spelta 220 which had different PHS tolerance,Were sub-cloned and the sequence alignment showed that many variations exist in B3 domain, especially,in the third intron and the fifth intron,including long fragment deletions. In addition, there also exist many SNPs located in the first and the sixth ex- ons,which changed the types of the amino acid. Based on the overlapping parts of the forward and reverse sequencing data, the partial sequences of the Vp-IB was joined into a whole length after their accuracy was judged, and named as SVp- 1Bc, SVp- 1Bd and SVp- 1Be, respectively. Sequence a- nalysis showed that SVp-1Bc has a deletions of 83 bp in the third intron,SVp-lBd has a deletion of 25 bp fragments in the same intron,and SVp-1Be has a deletion of 10 bp which located in the fifth intron region of the Vp-1B gene. In view of the relationship between Triticum aestivum L and Triticum spelta L, these allelic variations of the Vp-1B in Triticum spelta L may associate with seed dormancy.

关 键 词：穗发芽 斯卑尔脱小麦 Vp-1B 等位变异 

分 类 号：Q61[生物学—生物物理学]