西瓜噬酸菌Ⅳ型菌毛相关基因的功能研究  

Functional analysis of type Ⅳ pili-related genes in Acidovorax citrulli

作　　者：武珍[1] 邱晓静[1] 田艳丽[1] 刘凤权[1] 胡白石[1,2] 

机构地区：[1]南京农业大学植物保护学院/农作物生物灾害综合治理教育部重点实验室,江苏南京210095 [2]国家瓜类工程技术研究中心,新疆昌吉831100

出　　处：《南京农业大学学报》2013年第3期59-65,共7页Journal of Nanjing Agricultural University

基　　金：国家973计划项目(2009CB119200);全国科技支疆计划项目(201191133);国家公益性行业(农业)科研专项(201003066)

摘　　要：通过在NCBI上对西瓜噬酸菌(AC001)全基因组的比对,发现目前所知的Ⅳ型菌毛相关基因由18个基因组成,通过构建Ⅳ型菌毛部分基因的突变体,然后通过浸种试验进行致病力测定。结果发现,在突变的9个基因中,Aave_3550(PilE)、Aave_3156(PilZ)和Aave_4679(PilA)基因的突变对致病力影响明显。对致病力相关突变体进行相关功能分析发现,这些突变体的生长速度没有受到影响,而生物膜形成能力及游动能力明显下降,致病力减弱。构建互补菌株可使其功能部分恢复。上述结果证明:西瓜噬酸菌Ⅳ型菌毛与致病性相关,并明确了部分基因对致病性有影响。In the whole genome of Acidovorax citrulli the typeⅣ pili-related genes consist of 18 genes. In this study, we generated mutants of the type Ⅳ pili-related genes and virulence determination by seed transmission assays with melon. The results from the ex- periments showed Aave_3550 （ PilE）, Aave_3156 （ PilZ ） , and Aave_4679 （ PilA ） mutations of nine genes significantly affect the virulence. A functional analysis of these mutants found the growth rate was not affected, biofilm formation and swimming ability decreased significantly, and the mutants reduced virulence. Building complementary strain allows its function partially restored. Altogether, this study demonstrated that in Acidovorax citrulli type Ⅳ pill was associated with the pathogenicity, and clearly part of the type Ⅳ pili-related genes had a significant effect on pathogenicity.

关 键 词：西瓜噬酸菌 Ⅳ型菌毛 游动性 生物膜 致病性 

分 类 号：Q933[生物学—微生物学]