冠心病血瘀证差异表达基因及其启动子甲基化状态的初步研究  

Differentially Expressed Gene and Promoter Methylation Status of Coronary Heart Disease Patients with Blood Stasis Pattern

作　　者：王萍[1,2] 王丽萍[1] 黄献平[1] 喻松仁[1,2] 袁肇凯[1] 

机构地区：[1]湖南中医药大学中医诊断研究所 [2]江西中医学院

出　　处：《中医杂志》2013年第11期949-952,共4页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30973717)

摘　　要：目的探讨冠心病血瘀证差异表达基因及其启动子甲基化的情况。方法以家系冠心病血瘀证患者14例为观察对象,家系健康人7例为对照,采用表达谱芯片检测冠心病血瘀证差异表达基因。再收集冠心病血瘀证患者16例和健康人7例,选择其中2个差异基因进行启动子区甲基化特异性PCR(MS-PCR)研究,初步分析冠心病血瘀证差异表达基因及其启动子甲基化状态。结果表达谱芯片结果通过倍数变化值(FC)>3或FC<1/3、Call=P筛选,共获取差异表达基因26个,选择KLF5和LRP12基因进行MS-PCR,冠心病血瘀证与健康人各启动子甲基化率比较差异无统计学意义(P>0.05)。结论冠心病血瘀证差异基因KLF5和LRP12的启动子甲基化状态和健康人比较无明显区别。Objective To research the differentially expressed gene and promoter methylation status of coronary heart disease（CHD）with blood stasis pattern.Methods Fourteen family-related CHD patients with blood stasis pattern were selected as observation subjects and 7healthy persons were chosen as control.The differentially expressed genes of CHD with blood stasis pattern were detected by microarray.Another 16CHD patients with blood stasis pattern and 7healthy persons were chosen and 2differentially expressed genes were selected to conduct methylation-specific PCR（MS-PCR）of promoter region.The differentially expressed gene and promoter methylation status of CHD with blood stasis pattern was analyzed.Results A total of 26differentially expressed genes were obtained after screening the microarray results which fold change（FC）value〉3or FC value〈1/3and Call=P.The KLF5gene and LRP12gene were selected to conduct the MS-PCR.There was no significant difference between the CHD patients with blood stasis pattern and healthy persons in the promoter methylation rate（P〉 0.05）.Conclusion There is no significant difference between the CHD patients with blood stasis pattern and healthy persons in the promoter methylation status of differentially expressed genes KLF5and LRP12.

关 键 词：冠心病 血瘀证 差异基因 KLF5 LRP12 启动子 甲基化 

分 类 号：R259[医药卫生—中西医结合]