铊在真菌亚细胞中的分布特征  

Sub-cellular Distribution of Thallium in Fungus

作　　者：孙嘉龙[1,2] 肖唐付[1] 宁增平[1] 贾彦龙[1,3] 杨菲[1,3] 彭景权 

机构地区：[1]中国科学院地球化学研究所环境地球化学国家重点实验室,贵阳550002 [2]贵州省环境科学研究设计院,贵阳550002 [3]中国科学院研究生院,北京100039

出　　处：《北京工业大学学报》2013年第6期941-947,共7页Journal of Beijing University of Technology

基　　金：国家自然科学基金资助项目(41063005);中国科学院知识创新工程重要方向项目(kzcx2-yw-135)

摘　　要：将前期从铊污染区筛选得到的9株高耐受性真菌菌株用于微生物对铊的富集和亚细胞分布实验,采用酶解和差速离心法分离各亚细胞组分,并检测其中铊的质量分数.结果表明,生物富集量随着铊处理浓度上升而降低,其影响趋势与对生物量的影响趋势基本一致,最高可达7 189 mg/kg,最大富集系数为7.19.在亚细胞水平上,铊的富集优先顺序为:细胞质—细胞壁—细胞器.亚细胞水平的区隔化作用是真菌对铊的主要耐受机制,细胞质是赋存铊的主要场所(53.83%～79.45%).This paper focused on the sub-cellular distribution of thallium in several different fungus strains. The fungus strains were isolated from the Lanmuchang thallium-mineralised area in southwest Guizhou Province, and were used in the bio-concentration experiments. The sub-cellular supernatants including cytoplasmic, cellwall and cell organelles were seperated by the differential centrifuge after treated with snailase. Results show that the bio-concentration rate of thallium in the nine strains decreases with increasing thallium contents in the culture, the maximum at 7 189 mg/kg and the maximum bio- concentration factor （BCF） at 7. 19. In the sub-cellular supernatants detected by ICP-OES, the majority of thallium （up to 79.45% ） was found in cytoplasmic organelles, and the concentrations of thallium in the order of priority are as follows： eytoplasmic-cellwall-cell organelles. Cytoplasmic supernatant is apparently a major storage site for thallium, which is a possible detoxification of thallium by the fungus.

关 键 词：铊 微生物 真菌 亚细胞 分布 富集 

分 类 号：P593[天文地球—地球化学]