SA诱导对苹果叶片中PGIP基因表达的影响  

The Effects of SA Induction on the Expression of PGIP Gene in Apple Leaves

作　　者：瞿振芳[1] 符聪慧[1] 杨婷斐[1] 张军科[1] 

机构地区：[1]西北农林科技大学园艺学院,农业部西北地区园艺作物生物学与种质创制重点实验室,陕西杨凌712100

出　　处：《西北农业学报》2013年第3期103-109,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(30671447);陕西省科学技术研究发展计划(2011K01-43)

摘　　要：以抗病性不同的两个苹果品种秦冠和礼泉短富为材料,研究在叶面喷施不同浓度的SA对苹果叶片中PGIP基因表达水平的影响。结果表明,0.1和0.01mmol/L的SA叶面喷施处理对苹果叶片PGIP基因表达在0～96h、0～129d均有影响。在SA诱导处理后0～96h内,两种浓度的SA处理均可以促进秦冠叶片PGIP基因的表达,低浓度处理后,PGIP基因表达上调达到峰值的时间较长,峰值较高;高浓度的SA处理能提高礼泉短富叶片PGIP基因的表达水平,低浓度的SA处理抑制其表达。在SA诱导处理后0～129d内,前期(13～27d)秦冠叶片PGIP基因的表达维持在较高水平且低浓度SA处理促进效应大,后期(27～129d)表达量下降,促进效应不明显;高浓度的SA处理促进礼泉短富叶片PGIP基因表达,处理后第40天达到峰值,而低浓度的SA处理抑制其表达。With the material of Qinguan,and Liquan Fuji Spur apple plant,the effects of different concentration of salicylic acid（SA） spraying on PGIP gene expression of apple leaves were investigated.The results showed that 0.1 and 0.01 mmol/L SA treatments both promoted the PGIP gene expression of apple leaves in 0-96 h and 0-129 d after SA induction.After spraying treatment,both treatment of SA spraying promoted the PGIP gene expression from 0 to 96 h after induction in Qinguan leaves,under 0.01 mmol/L SA inducing treatment,the PGIP gene expression took more time to reach the peak but had higher peak value than that of the 0.1 mmol/L treatment.In Liquan Fuji Spur leaves the 0.1 mmol/L concentration treatment increased the PGIP gene expression level,but the 0.01 mmol/L concentration treatment had the inhibition effect in gene expression.After spraying treatment from 0 to 129 d,two concentrations of SA treatments could maintain the PGIP gene expression at a high level in Qinguan leaves during 13-27 days and the 0.01 mmol/L concentration had better promotion effect than the 0.1 mmol/L concentration,the expression level decreased during 27-129 days,and the promotion effect was not significant.In Liquan Fuji Spur leaves the 0.1 mmol/L concentration treatment promoted PGIP gene expression,reached the expression peak after 40 days,but the 0.01 mmol/L treatment inhibited in the PGIP gene expression.

关 键 词：苹果 PGIP基因 水杨酸 荧光实时定量PCR 

分 类 号：S661.1[农业科学—果树学]