甘肃省蔬菜根结线虫种群鉴定及其对主栽品种的致病性测定  

Species Identification of Root-knot Nematodes and Its Pathogenicity to Vegetable Varieties in Gansu Province

作　　者：叶德友[1] 王暄[2] 侯栋[1] 王晓巍[1] 

机构地区：[1]甘肃省农业科学院蔬菜研究所,兰州730070 [2]南京农业大学植物保护学院,南京210095

出　　处：《西北农业学报》2013年第3期188-193,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(31260434);国家大宗蔬菜产业体系(CARS-25-G-48);甘肃省自然科学基金(1208RJZA116)

摘　　要：对甘肃省6个蔬菜主产区的根结线虫发生与危害程度进行系统调查,根结线虫在各主产区均大量发生,土样中根结线虫检出率为58.02%。采用雌虫会阴花纹形态、Mdh-Est同工酶以及ITS-PCR技术,将采自甘肃省内13种蔬菜上的18个根结线虫种群鉴定为两类,在茄子与芹菜上发生花生根结线虫(Meloidogynearenaria),其余作物上均为南方根结线虫(Meloidogyne incognita),南方根结线虫占检测种群的88.89%。在田间进行9大类共23个蔬菜主栽品种和嫁接砧木的根结线虫抗性鉴定,参试辣椒品种、番茄品种"金鹏M215"和茄子砧木"托鲁巴姆"在田间未发生根结线虫病害,其余蔬菜各品种均为感病类型。Systematic investigation on occurrence and damage of root-knot nematodes was conducted for 6 of the main vegetable production areas in Gansu province.The results showed that vegetables diseases caused by root-knot nematodes have a high incidence and 58.02% soil samples were detected the occurrence of the pests.Based on female perineal pattern,Mdh-Est isozyme and ITS-PCR technology,18 Meloidogyne species were divided into two categories on 13 kinds of vegetables collected from Gansu.The root-knot nematodes detected in eggplant and celery are Meloidogyne arenaria,the nematodes from other crops are Meloidogyne incognita,which represents 88.89% of the tested populations.Field resistance test to the pests was carried out for a total of 9 categories and 23 vegetable cultivars and rootstocks.All pepper varieties,tomato variety Jinpeng M215 and eggplant rootstock Tuolubamu are resistant and the others are all susceptible.

关 键 词：蔬菜 根结线虫 种群鉴定 抗病鉴定 

分 类 号：S436.3[农业科学—农业昆虫与害虫防治]