不同粒径SiO_2致A549细胞毒性及氧化损伤作用实验研究  

Study on toxicity and oxidative damage in A549 cells induced by silica of different particulate size

作　　者：赵婉云[1] 刘贺荣[1] 黄敏[1] 周健[1] 杨惠芳[1] 

机构地区：[1]宁夏医科大学公共卫生学院,宁夏银川750004

出　　处：《工业卫生与职业病》2013年第3期153-157,共5页Industrial Health and Occupational Diseases

基　　金：宁夏回族自治区自然科学基金(NZ1098);自治区个人创新创业启动项目(170号)

摘　　要：目的探讨纳米SiO2及纳米/微米复合SiO2颗粒对A549细胞的毒性效应及氧化损伤作用。方法将纳米、微米、纳米/微米复合SiO2按照不同作用浓度(0、3.13、6.25、12.5、25.0、50.0、100、200μg/ml),对A549细胞进行染毒作用24h后,采用CCK-8法测定A549细胞的抑制率,DCFH-DA荧光染色法标记检测细胞活性氧(ROS)水平,微量酶标法测定细胞乳酸脱氢酶(LDH)活力。结果与阴性对照组比较,纳米、微米、纳米/微米复合组细胞抑制率均升高(P<0.05);且纳米/微米复合组始终高于纳米组及微米组。低浓度时微米SiO2对细胞抑制率高于纳米SiO2。随着浓度的增加,纳米组高于微米组(P<0.05)。各暴露组ROS水平则呈剂量-效应关系。同浓度纳米SiO2组高于微米SiO2组,且纳米/微米复合SiO2组高于同浓度纳米组及微米组。与阴性对照组比较,染毒组LDH水平升高,存在剂量-效应关系。同浓度纳米组高于微米组,纳米/微米复合组高于纳米组及微米组(P<0.05)。结论不同粒径SiO2均可引起A549细胞抑制率升高、ROS水平升高和细胞膜损伤。且纳米/微米复合SiO2组强于纳米组及微米组,同浓度纳米SiO2组高于微米组,说明粒径越小,对细胞损伤越大,而氧化损伤可能是纳米SiO2致细胞凋亡的一个途径。Objective To discuss nano silica and nano/micron silica composite induced toxicity and oxidative damage on A549 cells.Methods The nano,micro,and nano/micron silica composite with different concentrations（0,3.13,6.25,12.5,25.0,50.0,100,and 200 μg/ml）were exposed to A549 cells for 24 hours.The inhibition rates to A549 cells were observed by CCK-8 method.Levels of cell reactive oxygen species（ROS）were detected by DCFH-DA fluorescent marker.The changes of cell LDH were determined by trace enzyme standard method.Results In comparison with the control,inhibition rates of all nano,micro and nano/micron silica composite exposed groups raised（P0.05）;moreover,that of nano/micron silica composite group always higher than those of nano and micro groups.In low concentration the inhibition rate of micron silica was higher than that of nano silica.As the concentration increased,the rate of nano silica group turned to be higher than that of micro silica group（P0.05）.It is showed that each exposure group ROS levels in a dose-response relationship.When concentrations were same,those of nano silica groups were higher than those of micron silica groups,and those of nano/micron silica composite groups were higher than those of nano and micro groups.When compared with the negative controls,levels of LDH in exposed groups elevated showing dose-effect relationship.When the concentrations were same,levels of LDH in nano groups were higher than those of micron group,and those of nano/micron composite groups were higher than those of nano and micro groups（P0.05）.Conclusions Silica of different particle size can cause elevation of inhibition rate of A549 cell,elevation of active oxygen levels and injury of cell membrane.Moreover,the impact of nano/micron composite silica groups is stronger than those of nano and micro groups.In same concentration that of nano silica group are higher than that of micron group.The results shows that the smaller the particle size,the greater the cell damage;and oxidative damage may be the

关 键 词：纳米 微米SiO2 A549细胞 CCK-8 活性氧 LDH 

分 类 号：R135[医药卫生—劳动卫生]