丙烯腈对Jurkat细胞Bcl10蛋白及MEK1水平的影响  

Effect of acrylonitrile on Bcl10 protein of Jurkat cell and MEK1 levels

作　　者：李秀菊[1] 黄简抒[1] 王朋[1] 周元陵[1] 范卫[1] 刘淑朋[2] 

机构地区：[1]复旦大学附属金山医院,上海201508 [2]第二军医大学,上海200433

出　　处：《工业卫生与职业病》2013年第3期162-165,共4页Industrial Health and Occupational Diseases

基　　金：复旦大学附属金山医院第七期学科重点培养对象(2010-31)

摘　　要：目的研究丙烯腈(Acrylonitrile,AN)对Jurkat细胞信号转导中Bcl10蛋白及丝裂原活化蛋白激酶(MAP kinase kinase,MEK1)的影响,探讨AN免疫毒性的可能机制。方法采取体外细胞培养技术,将T淋巴细胞Jurkat细胞株分为空白对照组和AN染毒组,AN染毒组又分3组,染毒浓度分别为20、100和500μmol/L,分离脂筏并对脂筏内Bcl10蛋白进行分析;AN处理后4h收集蛋白样,用Western Blot方法检测MEK1及p-MEK1的水平。结果随AN染毒浓度增加,Bcl10蛋白总量不变,但定位多向脂筏下位组区集中;Western Blot结果显示,在一定染毒浓度内,MEK1蛋白分泌增加;超过一定浓度,MEK1蛋白分泌减少;而p-MEK1随着染毒浓度增加,其含量呈递减趋势,对照组和染毒组比较,差异有统计学意义(P<0.05)。结论AN可能通过破坏脂筏结构,导致Bcl10蛋白与脂筏分离,脂筏结构改变,使丝裂原活化的酪氨酸激酶信号转导受到抑制而产生免疫毒性。Objective To study the impact of acrylonitrile（AN）on Bcl10 protein and mitogen activated protein kinase in signal transmission and to explore the mechanism of its immune-toxicity in rat.Methods Jurkat cell lines were divided into control group and three AN exposed groups,i.e.low concentration group（20 umol/L）,medium concentration group（100 umol/L）and high concentration group（500 umol/L）,and cultivated in vitro.Lipid raft and Bc110 protein were separated and analyzed.Levels of MEK1 and p-MEK1 were detected by Western Blot.Results As AN concentration of exposure increased,the total amount of Bcl10 kept unchanged;however,the location concentrated mainly in lower area.Western Blot results showed that in a definite range of exposure the secretion of MEK1 protein increased.After the exposure exceeded the definite concentration,the secretion of MEK1 protein decreased,and the concentration of P-MEK1 increased as the exposure increased showing a tendency of gradual lowering.In comparison with the control,the difference showed statistical significant（P0.05）.Conclusions AN may produce the immune toxicity through damaging the lipid raft structure and causing the separation of Bcl10 protein and lipid raft,finally inhibiting the mitogen activated tyrosine kinase signal transmission pathway.

关 键 词：丙烯腈 Bcl10蛋白 MEK1 

分 类 号：R135.99[医药卫生—劳动卫生]