染矽尘大鼠支气管肺泡灌洗液中白介素含量及肺组织病理变化研究  

Study on the lung histo-pathological changes and interleukin in BALF of silica exposed rats

作　　者：王辉[1,2] 王瑞[1,2] 张玮[1,2] 徐伟生[3] 张海东[1,2] 

机构地区：[1]山东省职业卫生与职业病防治研究院 [2]济南大学山东省医学科学院医学与生命科学学院,山东济南250062 [3]山东中医药大学第二附属医院,山东济南250062

出　　处：《工业卫生与职业病》2013年第3期166-170,共5页Industrial Health and Occupational Diseases

基　　金：国家自然科学基金资助项目(30872094);山东省科技攻关项目(2008GG30002036)

摘　　要：目的观察染矽尘大鼠早期支气管肺泡灌洗液(BALF)中白细胞介素1(IL-1),白细胞介素6(IL-6),白细胞介素16(IL-16)的变化,探讨其与肺病理变化之间的关系。方法将168只Wistar大鼠随机分为4组:对照组,15mg/ml(低剂量)、30mg/ml(中剂量)、60mg/ml(高剂量)染矽尘组,每组42只。大鼠染尘采用1次性气管内注入矽尘法进行,各组均分时分段批量处死大鼠,收集BALF,采用酶联免疫吸附法(ELISA)测定其中IL-1、IL-6、IL-16浓度,同时观察肺组织病理变化。结果染尘时间和剂量均对BALF中的IL-1、IL-6、IL-16含量有明显影响(P<0.05或P<0.01)。高剂量组变化较为明显,在第1、3、7、14、21、28天时BALF中的IL-1含量分别为(133.55±27.0)、(154.50±42.11)、(136.43±54.71)、(198.0±35.5)、(103.38±8.9)、(127.50±31.9)nmol/L,明显高于对照组,差异有统计学意义(P<0.05);IL-6含量分别为(106.99±25.6)、(83.66±12.1)、(99.25±31.1)、(149.90±33.2)、(114.88±32.1)、(131.87±48.1)nmol/L,明显高于对照组,差异有统计学意义(P<0.05);IL-16含量分别为(1.95±0.47)、(2.30±0.65)、(2.20±0.74)、(3.14±0.45)、(2.14±0.60)、(2.67±0.76)nmol/L明显高于对照组,差异有统计学意义(P<0.05)。BALF中IL-1、IL-6、IL-16含量均在第14天出现高峰,与病理变化基本一致。结论染尘大鼠支气管肺泡灌洗液中IL-1、IL-6、IL-16含量变化与游离二氧化硅致大鼠肺纤维化发生发展密切相关。Objective To observe the changes of interleukin-1（IL-1）,interleukin-6（IL-6）,and interleukin-16（IL-16） in bronchoalveolar lavage fluid（BALF）,the pathological changes of lung tissue in the early stage of silica-exposed rats and to explore their correlations.Methods 168 Wistar rats used were randomly divided into four groups with 42 in each group,i.e.control group,low-,medium-and high-dose groups with silica exposure doses of 15,30 and 60 mg/ml,respectively.Silicotic rats were established by a single intratracheal instillation of silica.The rats in each group were sacrificed and BALFs were collected by the method of time-sharing and segmenting,meanwhile the pathological changes of lung tissue were observed by optical microscope.The levels of interleukin（IL-1,IL-6 and IL-16） were determined by enzyme-linked immunosorbent assay（ELLSA）.Results The levels of IL-1,IL-6 and IL-16 in BALF were affected significantly by the time of silica exposure and the doses of silica（P0.05 and P0.01）.More obvious changes were observed in the high-dose group compared to the control group.IL-l levels at the 1st,3rd,7th,14th,21st and 28th d were（133.55±27.0）,（154.50±42.11）,（136.43±54.71）,（198.0±35.5）,（103.38±8.9） and（127.50±31.9） nmol/L respectively,which were significantly higher than that of the control group（P0.05）.IL-6 levels at the 1st,3rd,7th,14th,21st and 28th d were（106.99±25.6）,（83.66±12.1）,（99.25±31.1）,（149.90±33.2）,（114.88±32.1） and（131.87±48.1）nmol/L respectively,which were also significantly different in comparison with the control group（P0.05）.IL-l6 levels at the 1st,3rd,7th,14th,21st and 28th d were（1.95±0.47）,（2.30±0.65）,（2.20±0.74）,（3.14±0.45）,（2.14±0.60） and（2.67±0.76） nmol/L,which were significantly different in comparison with the control group（P0.05）.The peak of IL-1,IL-6 and IL-16 levels reached at the 14th d,which was consistent with the pathological changes.Conclusions The content changes

关 键 词：矽肺 支气管肺泡灌洗液 白介素 

分 类 号：R134[医药卫生—劳动卫生]