紫花苜蓿对猪骨骼肌细胞肝X受体基因和蛋白表达的影响  

Effects of Alfalfa on the Expression of Liver X Receptors Gene and Protein in Pig Skeletal Muscle Cell

作　　者：陈颖[1] 王嘉福[1,2] 王芳[2] 袁恬[2] 张兆强[2] 冉雪琴[1] 

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州大学生命科学学院,贵州贵阳550025

出　　处：《中国畜牧兽医》2013年第5期30-34,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家科技支撑计划(2008BADB5B02);贵州省科技创新人才团队建设专项(黔科合人才团队2009-4006);贵州省优秀科技教育人才省长专项资金(黔省专合字2009-100)

摘　　要：为了探讨紫花苜蓿对猪骨骼肌细胞中肝X受体(liver X receptors,LXRs)表达的影响及其与胴体肉质的关系,采用免疫组织化学方法检测LXRs蛋白的分布,通过酶联免疫方法(enzyme-linked immunosorbent assay,ELISA)研究LXRs蛋白总量的改变,以荧光定量PCR方法分析猪骨骼肌细胞中LXRs基因mRNA的表达变化。结果表明,LXRs定位于骨骼肌细胞的细胞质、细胞膜和细胞核内;与对照组相比,日粮中添加4%紫花苜蓿能使LXRs基因和蛋白表达量明显上升(P<0.05),并使猪的瘦肉率降低(P>0.05),肌内脂肪含量显著增高(P<0.05),同时大理石纹评分较好(P<0.05),滴水损失下降(P<0.05),细胞间隙变小(P<0.05);添加6%紫花苜蓿使LXRs基因和蛋白表达量上升(P<0.05),但低于4%紫花苜蓿组(P>0.05),同时大理石纹评分及失水率增高(P<0.05),板油重下降(P<0.05)。结果提示,紫花苜蓿添加量的不同对猪肉品质的作用效果不同,为紫花苜蓿在养猪业的科学应用奠定了理论基础。To investigate the effects of alfalfa on the expression of liver X receptors（LXRs） gene and protein in pig skeletal muscle cell,and the influence on carcass and meat quality in pig,LXRs protein of longissimus muscle in pig was detected by immunohistochemistry（IHC） and enzyme-linked immunosorbent assay（ELISA） methods.The mRNA expression level was quantitated by Real-time quautitative PCR system.The results indicated that LXRs signal was located in the cell nucleus,cytoplasm and cell membrane.Compared with the control group,adding 4% alfalfa to daily ration could elevate the expression levels of LXRs gene and the protein（P0.05）,decrease leanness（P0.05） and significantly increase intramuscular fat content together with a better marbling score,less water loss and smaller intercellular space（P0.05）.In 6% alfalfa group,marbling score and water lose increased,while suet weight decreased（P0.05）.A higher expression of LXRs gene and protein in 6% alfalfa group were detected,but were lower than 4% alfalfa group（P0.05）.It suggested that different additive amounts of alfalfa might result in different degree of improvement on meat quality for future usage in pig industry.

关 键 词：紫花苜蓿 肝X受体 免疫组化 酶联免疫吸附试验 荧光定量PCR 

分 类 号：Q786[生物学—分子生物学]