猪钙蛋白酶抑制蛋白基因外显子9多态性检测及其对山西白猪背膘厚的影响  

Study on the Polymorphisms of CAST Gene Exon 9 and its Effect on Backfat Thickness in Shanxi White Pig

作　　者：赵先萍 黎威[2] 王彦玲[2] 贺晓丽[2] 高鹏飞[2] 郭晓红[2] 李步高[2] 曹果清[2] 

机构地区：[1]山西省畜牧兽医学校,山西太原030024 [2]山西农业大学动物科技学院,山西太谷030801

出　　处：《中国畜牧兽医》2013年第5期161-165,共5页China Animal Husbandry & Veterinary Medicine

基　　金：山西省科技攻关项目(991016;021043;20080311031);山西省留学人员科技活动择优资助项目(614193);山西省高等学校优秀青年学术带头人支持计划;山西省大学生科技创新项目

摘　　要：本研究采用PCR-SSCP方法检测了杜洛克猪、大白猪、长白猪、山西白猪、山西黑猪和马身猪6个品种共416头个体的钙蛋白酶抑制蛋白(calpastatin,CAST)基因外显子9的多态性,在扩增片段内检测到5个SNPs,分别是第37位的T→G突变、150位的A→G突变、167、193和256位点的C→T突变,其中第193和256位点的C→T突变位于外显子9内,为错义突变,分别引起苏氨酸→异亮氨酸和苏氨酸→蛋氨酸的转变;5个SNPs位点形成A、B、C、D、E、F和G 7种单倍型,其分布与猪的经济类型相一致。在引入猪种大白猪和杜洛克猪群体中,有3种单倍型A、B和C,单倍型B频率较高,分别为0.40和0.53;长白猪群体中只有B和C 2种单倍型,单倍型C频率较高,为0.72;马身猪除含有A、B、C 3种单倍型外,还含有单倍型E,频率为0.16。统计分析结果表明,CAST基因单倍型类型对山西白猪6月龄活体背膘厚无显著影响。A total of 416 pigs from Duroc,Large White,Landrace,Shanxi White,Shanxi Black and Mashen were used to detect the polymorphisms of calpastatin（CAST） gene exon 9 by PCR-SSCP.Five SNPs were detected in the amplified region,which were T37G,A150G,C167T,C193T and C256T,of them,the mutations of C193T and C256T located in the CAST gene exon 9 were missense mutation,which gave rise to the changes from threonine to isoleucine and from threonine to methionine,respectively.Seven haplotypes of A,B,C,D,E,F and G were detected in the examined populations.The distributions of the haplotypes in different pig breeds were anastomosed with the pig economic types.There were three haplotypes of A,B and C in the imported pigs of Large White and Duroc,and the frequency of haplotype B was greater than those of A and C,which were 0.40 in Large White and 0.53 in Duroc.Only two haplotypes of B and C were detected in Landrace,the frequency of haplotype C was greater than that of haplotype of B.Except of the haplotypes of A,B,and C,a new haplotype of E was detected in Mashen pig population,the frequency was 0.16.The haplotypes had no significant effect on the backfat thickness measured alive in Shanxi White pigs.

关 键 词：猪 PCR-SSCP 钙蛋白酶抑制蛋白基因 背膘厚 

分 类 号：S813.3[农业科学—畜牧学]