康斯特液与低钾右旋糖酐液对肺动脉内皮细胞功能的影响  

Effects of HTK and Perfadex Solution on the Function of Pulmonary Artery Endothelium

作　　者：何立术[1] 周琦云[1] 胡应学[1] 冯志强[1] 黄强信[1] 龚坚[2] 李世康[1] 

机构地区：[1]广西医科大学第一附属医院心胸外科,530021 [2]广西医科大学第一附属医院急诊科,530021

出　　处：《天津医药》2013年第5期475-477,共3页Tianjin Medical Journal

基　　金：广西医疗卫生重点研究课题资助项目(项目编号:重200827)

摘　　要：目的探讨康斯特保护液(HTK液)与低钾右旋糖酐液(Perfadex液)低温保存对猪肺动脉内皮细胞功能的影响。方法 9只新鲜猪肺,每只取小叶间动脉(直径500～700μm)切成6条,长2mm,54条血管随机分成3组,每组18条。于4℃条件下分别用重碳酸盐缓冲液(KH液,对照组)、HTK液(实验A组)和Perfadex液(实验B组)保存4h,检测在消炎痛(7μmol/L)、N-硝基-L-精氨酸(300μmol/L)及氧合血红蛋白(20μmol/L)作用下,前列腺素F2α(1×10-7.5mol/L)引发的血管收缩反应,缓激肽、非受体介导钙离子载体引发的血管舒张反应。结果前列腺素F2α引起的血管环最大收缩强度,缓激肽及非受体介导钙离子载体引发的血管环最大舒张反应,对照组及各实验组差异均无统计学意义(P>0.05)。结论 HTK液、Perfadex液与KH液低温保存对内皮依赖性超极化因子介导的内皮源性舒张功能的影响无明显差异。Objective To study the effect of HTK solution and perfadex solution on the function of pulmonary ar- tery endothelium. Methods Fifty-four porcine pulmonary rings（2 mm long） were randomly divided into three groups, in- cluding control group （n=18） incubated in Krebs-Henseleit （KH） at 4 ℃ for 4 hours, group A （n=18） incubated in HTK solution and group B （n=15） incubated in perfadex solution at 4 ℃ for 4 hours. The vasoconstriction responses induced by in- domethacin （7 μmol/L）, N-nitro-L-arginine（300 μmol/L）, oxyhemoglobin （20 μmol/L） and prostaglandin F2α（ 1 × 10^-7.5mol/L） were detected. The vasodilator responses induced by bradykinin and non-receptor-mediated calcium ionophore were also de- tected in three groups. Results The maximum contraction of the vascular ring was caused by prostaglandin F2α. The maxi- mum relaxtion responses of the vascular ring were caused by bradykinin and non-receptor-mediated calcium ionophore. There were no significant differences between three groups （P 〉 0.05）.Conclusion There was no significant difference in en- dothelium-derived hyperpolarizing factor （EDHF）-mediated relaxation of porcine puhnonary artery between HTK,peffadex and KH solutions.

关 键 词：肺动脉 内皮细胞 生物因子 右旋糖酐类 康斯特液 

分 类 号：R977[医药卫生—药品]