创伤脓毒症患者血清小分子蛋白组变化研究  

Changes in the serum low molecular weight proteome of patients with posttraumatic sepsis

作　　者：冯凯[1] 李瑛[2] 郑燕华[1] 卞晓星[2] 张军[1] 沈文明[2] 张建中[1] 陆洁[2] 李建忠 郑琦涵[2] 秦亚亚[1] 尹进南[2] 赵晓成[2] 岳茂兴 

机构地区：[1]解放军第306医院病理实验科,北京100101 [2]常州市武进人民医院 [3]特种医学中心

出　　处：《中华急诊医学杂志》2013年第5期476-481,共6页Chinese Journal of Emergency Medicine

基　　金：常州市社会发展科技计划（CS20102002）;武进科技局重大课题（WS2010007）

摘　　要：目的观察创伤脓毒症患者血清小分子蛋白组学特征，并观察患者血清小分子蛋白质组随脓毒症进程的变化规律。方法基质辅助激光解吸电离飞行时间质谱（matrix．assistedlaserdesorption／ionizationtimeofflightmassspectrometry，MALDI-TOF-MS）技术对血清样本进行蛋白质谱分析，比较24例见刊人、24例创伤患者、12例创伤后脓毒症患者的血清小分子蛋白水平差异，经聚类分析，揭示创伤脓毒症患者的蛋白组学特性。通过对创伤脓毒症患者发病过程中血清小分子蛋白水平的连续观察，通过聚类分析，揭示创伤脓毒症发病进程的组学特征。结果创伤脓毒症患者血清小分子蛋白谱与创伤对照组和健康对照组比较，均存在明显差异，发现了28个差异蛋白分子，处在不同创伤脓毒症发展阶段的患者的血清小分子蛋白谱之间也存在着明显差别。结论血清小分子蛋白谱的波动可以较早诊断创伤脓毒症，并可通过血清小分子蛋白谱的变化观察创伤脓毒症的严重程度。Objective To observe the features of the serum low molecular weight proteome in pateints subjected to posttraumatic sepsis. Methods Serum proteomic spectra were generated by matrix- assisted laser desorption/ionization time of flight mass spectrometry （MALDI-TOF-MS） technology. Serum samples of 24 healthy controls, 24 traumatic patients and 12 posttraumatie sepsis patients were analyzed by comparing the level of low molecular weight proteins. And hierarchical cluster analysis was used to reveal the group characteristics in the process of the pathogenesis of posttraumatic sepsis by continuous observation of the serum level of low molecular weight proteins. And hierarchical cluster analysis was used to reveal the group characteristics in the process of the pathogenesis of posttraumatic spesis by continuous boservation of the serum level of low molecular weight proteome. Results Comparing peak intensities of serum from traumatic patients, posttraumatic sepsis patients with those from healthy controls, mean intensity differed significantly for 28 peaks. And there is a clear difference in the serum low molecular weight proteome profiling of patients between different stages of sepsis development. Conclusions The spectrum of the fluctuations in the serum low molecular weight proteome can be used for earlier diagnosis of sepsis, and severity of sepsis can be predicted by observing the changes of serum low molecular weight proteome profiling.

关 键 词：基质辅助激光解吸电离飞行时间质谱 创伤脓毒症 生物标志分子 

分 类 号：R459.7[医药卫生—急诊医学]