人参皂甙Rg1对大鼠关节软骨细胞凋亡的拮抗作用  

Ginsenoside Rg1 protects articular chondrocytes from apoptosis in rats

作　　者：吴东迎 惠宇坚 左强 范卫民 

机构地区：[1]南京医科大学第一附属医院骨科,江苏省210029

出　　处：《江苏医药》2013年第9期996-998,共3页Jiangsu Medical Journal

基　　金：国家自然科学基金(81000800);江苏省自然科学基金(BK2011843)

摘　　要：目的探讨人参皂甙Rg1对软骨细胞凋亡的影响。方法正常大鼠关节软骨细胞分为三组:A、B组用白细胞介素1β(IL-1β)10ng/ml作用24h,建立软骨细胞凋亡模型;B组事先加入10μg/ml的人参皂甙Rg1保护2h;C组作为空白对照。TUNEL法检测人参皂甙Rg1对软骨细胞凋亡的影响;Western blot检测人参皂甙Rg1对凋亡软骨细胞基质金属蛋白酶13(MMP-13)和基质金属蛋白酶组织抑制剂1(TIMP-1)蛋白表达的影响。结果 A、B和C组细胞凋亡率分别为(37.30±4.05)%、(10.30±1.08)%和(2.20±1.93)%,B组胞凋亡率低于A组(P<0.05)。与A组比较,B组软骨细胞TIMP-1的表达增加,MMP-13的表达降低(P<0.05)。结论人参皂甙Rg1具有拮抗大鼠关节软骨细胞凋亡的作用。Objective To investigate the influence of Ginsenoside Rgl against interleukin-1 beta （IL-1β） - induced apoptosis in rat articular chondrocytes. Methods The articular chondrocytes were divided into three groups. The ehondrocytes in groups of A and B were exposed to IL-1β 10 ng/ml for 24 h to create apoptosis model. Ginsenoside Rgl 10 μg/ml was added at 2 h before IL-1β administration in group B. Group C was taken as blank control. The chondrocyte apoptosis was assessed by TUNEL staining and the expressions of MMP-13 and TIMP-i were detected by Western blot. Results The chondrocyte apoptosis rates in groups of A,B and C were （37. 30±4.05）%, （10. 30±1.08）% and （2.20± 1.93）%, respectively, which was lower in group B than that in group A（P〈0. 05）. Compared to group A, the expression of TIMP-1 was higher, but that of MMP-13 was lower, in group B （P〈0. 05）. Conclusion Ginsenoside Rgl can protect articular chondrocytes from apoptosis induced by IL-1β in rats.

关 键 词：人参皂甙RG1 软骨细胞 细胞凋亡 

分 类 号：R681[医药卫生—骨科学]