人参皂苷Rh2对肝癌细胞表皮生长因子受体表达水平的影响  

Ginsenoside Rh2 inhibits the proliferation of hepatocellular carcinoma cells via the epidermal growth factor receptor pathway

作　　者：谢炜[1] 罗阔[2] 韩萍[2] 

机构地区：[1]重庆市中山医院普外科,400010 [2]重庆市中山医院肿瘤科,400010

出　　处：《免疫学杂志》2013年第6期490-493,共4页Immunological Journal

摘　　要：目的研究人参皂苷Rh2对肝癌细胞表皮生长因子受体(EGFR)表达的影响,以期为人参皂苷Rh2的抗肿瘤机制提供实验证据。方法采用实时定量荧光PCR、Western blot、MTT法、荷瘤小鼠模型研究人参皂苷Rh2对肝癌细胞EGFR的表达影响,以及对肝癌细胞的生长的抑制作用。结果人参皂苷Rh2对肝癌细胞EGFR的表达具有明显的抑制作用,并且这种抑制作用具有质量浓度依赖性;人参皂苷Rh2可在体外及体内抑制肝癌细胞的增殖。结论人参皂苷Rh2抑制肝癌细胞的增殖有可能是通过EGFR通路发挥作用。To investigate the effects of ginsenoside Rh2 on the expression of epidermal growth factor receptor （EGFR） of hepatocellular carcinoma cells, we employed the real-time quantitative fluorescent PCR and Western blot to determine the expression of EGFR mRNA and protein, and using the MTT and tumor-bearing mice model to investigate the inhibition of ginsenoside Rh2 on hepatocellular carcinoma cells proliferation. Results showed that ginsenoside Rh2 could significantly inhibit liver cancer cells EGFR expression in a concentration-dependent manner, and then inhibited the proliferation of liver cancer cell in vitro and in vivo. In conclusion, EGFR pathway may play a key role in the inhibitory effects of ginsenoside Rh2 on the proliferation of hepatoma cells.

关 键 词：人参皂苷 肝癌 表皮生长因子受体 

分 类 号：R285.5[医药卫生—中药学] R735.7[医药卫生—中医学]