系统性红斑狼疮患者单核细胞中TLR-4、IRAK-M的表达及意义  

The expression of TLR-4 and IRAK-M in monocytes of patients with systemic lupus erythematosus and its significance

作　　者：张文兰[1] 胡同平[1] 王慧[1] 庞春燕[1] 任艳玲[1] 赵剑波[1] 尹芳蕊[1] 王永福[1] 

机构地区：[1]包头医学院第一附属医院中心实验室,014010

出　　处：《免疫学杂志》2013年第6期510-513,共4页Immunological Journal

基　　金：内蒙古自治区高等学校科学研究项目(NJZY12219)

摘　　要：目的研究SLE组和健康对照组外周血单核细胞中TLR-4、IRAK-M的mRNA表达情况和血清中TNF-α、IL-6、IL-10水平。方法采用qRT-PCR技术,检测SLE组和健康对照组单核细胞中TLR-4、IRAK-M的mRNA表达量;同时采用ELISA方法检测血清中TNF-α、IL-6、IL-10水平;相关性分析采用Pearson或Spearman。结果活动期SLE组TLR-4的mRNA表达量明显高于稳定期SLE组和健康对照组(P<0.05);SLE组IRAK-M的mRNA表达量明显低于健康对照组(P<0.05),活动期SLE组IRAK-M的mRNA表达量明显低于稳定期SLE组(P<0.05);SLE组TNF-α、IL-6、IL-10水平均高于健康对照组(P<0.05),活动期SLE组TNF-α、IL-6、IL-10水平均高于稳定期SLE组(P<0.05);相关分析显示:TLR-4的mRNA表达量、TNF-α、IL-6、IL-10的表达水平均与SLEDAI评分呈正相关(P<0.05),而IRAK-M的mRNA表达量与SLEDAI评分呈负相关(P<0.05)。结论 TLR-4、IRAK-M在SLE的发病中有一定意义;临床可以通过对TLR-4、IRAK-M的mRNA表达量、TNF-α、IL-6、IL-10的表达水平的监测来作为判断SLE疾病活动和预后的指标。This study designed to compare the expression of TLR-4 and IRAK-M in monocytes and the levels of TNF-α、IL-6、IL-10 in serum between SLE patients and healthy controls. The qRT-PCR was performed formRNA measurement and ELISA was used for cytokine quantification. The Pearson or Spearman correlation between TLR-4, IRAK-M, TNF-α、IL-6、IL-10 and SLEDAI were computed to explore their roles in the pathogenesis ofSLE. The result showed that the mRNA expression of TLR-4 in active group was significantly higher than that of stable group and healthy controls （P 〈 0.05）; the mRNA expression of IRAK-M in SLE patients was significantlylower than that of healthy controls （P〈 0.05）; and the mRNA expression of IRAK-M in active group was significantly lower than that of stable group （P〈 0.05）. The levels of TNF-α、IL-6 and IL-10 in serum of SLE patients weresignificantly higher than those of healthy controls （P〈 0.05）; and the levels of TNF-α、IL-6 and IL-10 in active group were significantly higher than those of stable group （P〈 0.05）. Correlation analysis showed that the levels of TLR-4, TNF-α, IL-6 and IL-10 were positively correlated with SLEDAI （P〈 0.05）, but the mRNA expression of IRAK-M was negatively correlated with SLEDAI （P 〈 0.05）. This study indicated that TLR-4 and IRAK-M playcertain roles in the pathogenesis of SLE. We can evaluate the SLE disease activity and prognosis by determining TLR-4, IRAK-M, TNF-α, IL-6 and IL-10.

关 键 词：系统性红斑狼疮 TOLL样受体4 白细胞介素-1受体相关激酶M 细胞因子 SLEDAI评分 

分 类 号：R593.241[医药卫生—内科学]