中国部分地区蝙蝠携带病毒的宏基因组学分析  

Metagenomic analysis of bat virome in several Chinese regions

作　　者：杨凡力[1,2] 王意银[3] 郑文成 何彪[2] 江廷磊[5] 李莹莹[2] 夏乐乐[2] 冯烨[2] 范泉水[3] 涂长春[2] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]军事医学科学院军事兽医研究所,吉林长春130122 [3]成都军区疾病预防控制中心,昆明云南650118 [4]湖南省动物卫生监督所,长沙湖南410007 [5]东北师范大学吉林省动物资源保护与利用重点实验室,吉林长春130024

出　　处：《生物工程学报》2013年第5期586-600,共15页Chinese Journal of Biotechnology

基　　金：NSFC云南省联合基金(No.U1036601)资助~~

摘　　要：蝙蝠携带有60多种病毒,其中许多对人有高度致病性。为了解中国蝙蝠携带病毒的自然本底、蝙蝠病毒的多样性和挖掘潜在的病毒病原,通过基于Solexa高通量测序的病毒宏基因组学技术对从吉林、云南、湖南采集的蝙蝠组织进行病毒组学研究,获得了11 644 232条读长(Reads),并拼接出44 872条重叠序列(Contig)。通过核酸序列注释发现,其中8.2%(4 002/44 872)的重叠序列与病毒相关,能进一步注释到36个病毒科,包括19种脊椎动物病毒、6种植物病毒、4种昆虫病毒和4种噬菌体。通过对重叠序列的遗传进化分析、多序列比对显示,被注释为细小病毒、腺联病毒、博卡病毒、腺病毒、小双节RNA病毒等的重叠序列与已知病毒相似,部分序列却又呈现出明显的序列差异。通过对腺病毒和博卡病毒进一步的PCR扩增证实了此研究方法可靠。旨在了解我国蝙蝠携带病毒组的构成,对建立高效的野生动物源人兽共患病的监测方法提供参考。Bats are important reservoir animals and more than 60 viruses have been identified in bats with many of them highly pathogenic to human. In order to understand the natural background, genetic diversity of bat viruses in China and discover potential viral pathogens, Solexa sequencing based viral metagenomics focusing on bats tissues was established and to analyze the virome of bats collected from Jilin, Yunnan and Hunan province. By Solexa sequencing, 116 442 324 useful reads were obtained and assembled into 4 872 contigs, of which 8.2% （4 002/4 4872） were annotated to 36 viral families, including 19 vertebrate virus families, 6 plant virus families, 4 insect virus families and 4 phages. Further contigs analyses showed that some adenovirus, bocavirus, picobirnavirus, parvovirus contigs sequences were similar with known viruses. However, part of them shared limited identities to these viruses implying the discovery of new viruses. Moreover, PCR validation of adenovirus and bocavirus confirmed the results obtained by viral metagenomics. This study aimed to understand bat virome in China by viral metagenomics and could be helpful to establish effective surveillance on wildlife-associate zoonoses.

关 键 词：病毒宏基因组学 蝙蝠 病毒组 高通量测序 

分 类 号：Q939.4[生物学—微生物学]