主要组织相容性复合体(MHC)B-LβⅡ基因Hin1Ⅰ位点多态性对鸡免疫功能的影响  

The Effects of Chicken Major Histocompatibility Complex (MHC) B-LβⅡ Gene Hin1Ⅰ Locus Polymorphism on Immune Function

作　　者：朱晓庆[1] 谷新利[1] 乔海博[1] 

机构地区：[1]石河子大学动物科技学院,石河子832003

出　　处：《农业生物技术学报》2013年第5期570-578,共9页Journal of Agricultural Biotechnology

摘　　要：鸡主要组织相容性复合体(MHC)基因具有高度多态性和稳定性,,与机体抗病性和免疫应答密切相关,是家禽抗病育种研究中的优选标记基因。本研究采用PCR-RFLP法,分析170只良凤青脚麻鸡(Gallus gallus)MHC B-Lβ(MHC classⅡ)基因第2外显子Hin1Ⅰ位点的多态性及其对鸡部分免疫性状(淋巴细胞活性,新城疫(ND)抗体浓度,γ干扰素(IFN-γ)、白介素4(IL-4)和白介素12(IL-12)含量)的影响,探讨MHC B-LβⅡ基因第2外显子多态性及其对鸡免疫功能的影响。结果表明:MHC B-LβⅡ基因第2外显子经Hin1Ⅰ酶切后检测到A、B两种等位基因和AA、AB、BB3种基因型,其等位基因频率分别为0.553和0.447,基因型频率分别为0.235、0.636和0.129;AA、AB基因型个体淋巴细胞活性、新城疫抗体浓度、IFN-γ和IL-4含量高于BB基因型;IL-12含量在3种基因型间无明显差异。推测MHC B-LβⅡ基因第2外显子Hin1Ⅰ位点多态性对鸡免疫功能有一定影响,可作为鸡抗病力性状的一个候选基因。The major histocompatibility complex （MHC） gene in chicken, which has a high degree of polymorphism and stability, and closely associated with resistance and immune response, is the preferred marker gene for disease resistance research in poultry breeding. In order to explore the distribution of polymorphism in MHC B-LβⅡ （MHC class Ⅱ ） gene exon 2 and the effects of its polymorphism on immune function, PCR- RFLP technique was applied to analyze the polymorphism of MHC B-Lβ Ⅱgene exon 2 Hinl I locus and its impact on some immune traits （lymphocyte proliferation, antibody response kinetics to Newcastle disease （ND） vaccine, concentration of Interferon-y（1FN-7）, Interleukin-4（IL-4） and Interleukin-12（IL-12）） in 170 Liangfeng Cyan-shank partridge chickens（Gallus gallus）. The results showed that two alleles （A, B） and three genotypes （AA, AB and BB） were detected by digestion of restriction endonuclease Hinl I, and these frequencies were 0.553, 0.447 and 0.235, 0.636 and 0.129, respectively; and the lymphocyte proliferation, concentration of ND antibody, IFN-y and IL-4 of genotype AA, AB were higher than that of genotype BB, while there were no significant differences between AA, AB and BB type in the concentration of IL-12. The results indicated that MHC B-LβⅡ gene HinlⅡ locus polymorphism has a certain influence on the immune function of chicken, and it can be treated as a candidate gene for disease resistance.

关 键 词：鸡 MHC B-Lβ Ⅱ基因 PCR-RFLP 免疫功能 

分 类 号：S813[农业科学—畜牧学]