稻瘟病菌一个假定苯基丙酮单加氧酶基因(MoPAMO1)的功能分析  

Functional Analysis of a Putative Phenylacetone Monooxygenase Gene(MoPAMO1) in Magnaporthe oryzae

作　　者：吴丽民[1,2] 卞文印[1] 胡芳辉[1] 连永乐[1] 王宗华[1] 鲁国东[1] 

机构地区：[1]福建农林大学生物农药与化学生物学教育部重点实验室,福州350002 [2]福建生物工程职业技术学院,福州350002

出　　处：《农业生物技术学报》2013年第5期595-602,共8页Journal of Agricultural Biotechnology

基　　金：国家自然基金项目(No.30871616;30671348);高等学校博士学科点专项科研基金(No.20113515110002)

摘　　要：对稻瘟病菌定殖过程中致病相关基因的功能研究有助于进一步揭示稻瘟病菌的致病机理。本研究通过生物信息学和分子遗传学方法对稻瘟病菌假定苯基丙酮单加氧酶基因(MoPAMO1)的功能进行了分析。结果显示,MoPAMO1编码一个假定的苯基丙酮单加氧酶,系统进化分析表明,它与同属丝状真菌的轮枝孢菌对应蛋白的关系最为接近。MoPAMO1基因在病菌侵染过程中被诱导表达,其缺失突变体的产孢量减少,但生长速度和致病性与野生型相比没有显著差异(P<0.01)。表明该基因参与了稻瘟病菌的产孢过程。由于稻瘟病菌数据库中存在另外5个可能功能冗余的苯基丙酮单加氧酶基因,致使该基因与病菌营养生长和致病性的关系尚无法明确。本研究为进一步揭示MoPAMO1的生物学功能提供了基础数据。Functional analysis of the pathogencity related genes during the colonization process of rice blast fungus Magnaporthe oryzae will contribute to uncovering its molecular pathogenesis mechanism. In this study, the function of putative phenylacetone monooxygenase gene （MoPAMO1） in M. oryzae was analyzed through bioinformatics and molecular genetics. The results showed that MoPAMOI encoded a putative phenylacetone monooxygenase, which is most close to the ortholog of filamentous fungi Verticillium dahliae according to the phylogenetic analysis. The expression of MoPAMOI was induced during the infection process of M. oryzae. MoPAMO1 deletion mutants displayed fewer conidia, while the vegetative growth and patheogencity showed as normal as the wild type strain（P〈0.01）. Our results indicated that MoPAMO1 was involved in conidiation ofM. oryzae, and its relationship with vegetative growth and pathogenicity was still undefined since there were other 5 putative phenylacetone monooxygenase genes, which may be functional redundancy. Our research establishes a foundation for further clarifying the biological function of MoPAMO1.

关 键 词：稻瘟病菌 MoPAMO1 功能分析 产孢 

分 类 号：S816.7[农业科学—饲料科学]