识别HIV-1 P_(24)蛋白的单克隆抗体杂交瘤细胞株的建立  

Establishment of Hybridoma Producing Monoclonal Antibodies Specific for HIV-1 P24 Core Protein

作　　者：尹红章[1] 张碧如[1] 尹一鸣[1] 蒋先敏[1] 赵莉[1] 张孝月 程明[1] 

机构地区：[1]卫生部北京生物制品研究所,北京100024

出　　处：《单克隆抗体通讯》1991年第4期31-33,共3页

摘　　要：以细胞培养的HIV-1全病毒抗原免疫,通过鼠-鼠杂交瘤技术获得3株分泌单克隆抗体(以下简称单抗)的杂交瘤细胞系(21A、51G、47B)。免疫印迹结果显示3株单抗均能识别病毒的核心元原-P_(24)蛋白。ELISA阻断抑制试验提示,3株单抗分别识别两个不同的抗原决定簇。进而为HIV-1病毒抗原的研究和制备以双抗体夹心法检测P_(24)抗原的试剂打下基础。Three mouse hybridomas which produce monoclonal antibodies against the p24 core protein of HHIV-1 were isolated. Cross-competition assay demonstrated that two closely related epitopes might be identified by these antibodies. They might be used for the study of HIV-1 core antigen and selective detection of p24 polypeptide by a solid-phase sandwich EIA.

关 键 词：艾滋病毒 核心蛋白 单克隆抗体 

分 类 号：R392－33[医药卫生—免疫学]