激光扫描共聚焦显微镜样品制备的改进方法用于8种纤毛虫表膜的观察  

The improvement of sample preparation for the confocal laser scanning microscopy used for the observation of eight ciliate membranes

作　　者：陈晶[1] 肖莉杰[1] 余丽芸[1] 

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,黑龙江大庆163319

出　　处：《电子显微学报》2013年第2期163-167,共5页Journal of Chinese Electron Microscopy Society

基　　金：黑龙江省青年科学基金资助项目(No.QC2009C115)

摘　　要：采用KMnO4作为固定剂,对传统的免疫荧光细胞化学技术进行改进,将制备好的样品用激光扫描共聚焦显微镜进行连续断层扫描和三维重建,清晰地呈现了8种纤毛虫的表膜以及纤毛模式,为原生动物纤毛虫表膜研究提供了一种可供借鉴的样品制备方法。By using KMnO4 as fixative,the traditional immune-fluorescence cytochemistry technology can be improved.The prepared sample was studied by continue section scanning and three dimensional reconstruction with confocal scanning laser microscopy.The eight ciliate membranes and the Ciliate mode can be clearly observed.This method provides a reference for the sample preparation of protozoon ciliate membranes.

关 键 词：纤毛虫 激光扫描共聚焦显微镜 表膜 

分 类 号：Q-336[生物学]