瑞芬太尼和芬太尼对大鼠脊髓背角神经元NMDA受体通道电流的影响  

Effects of remifentanil and fentanyl on N-methyl-D-aspartate receptor currents in rat spinal cord dorsal horn neurons

作　　者：李思思[1] 郁葱[1] 罗玉琳[1] 

机构地区：[1]口腔疾病与生物医学重庆市重点实验室重庆医科大学附属口腔医院麻醉科, 400015

出　　处：《中华麻醉学杂志》2013年第3期299-301,共3页Chinese Journal of Anesthesiology

基　　金：基金项目：重庆市卫生局科研项目（2010-2.227）

摘　　要：目的探讨瑞芬太尼和芬太尼对大鼠脊髓背角神经元NMDA受体通道电流的影响。方法采用全细胞膜片钳技术记录NMDA受体通道电流。原代培养的E14SD大鼠脊髓背角神经元（DH细胞）30个，采用随机数字表法，将其分为3组（n=10）：瑞芬太尼组（R组）、芬太尼组（F组）、对照组（C组）。4nmol／L瑞芬太尼（R组）、10μmol／L芬太尼（F组）灌流DH细胞60min后洗脱。于给药后即刻（T0）、药物作用15min（T1）、30min（L）、45min（T3）、60min（T4）、洗脱后15min（L）、30min（L）时记录NMDA受体通道电流。结果与C组比较，F组各时点NMDA受体通道峰电流差异无统计学意义，R组T0、T1时NMDA受体通道峰电流差异无统计学意义（P〉0．05），T2-T6时NMDA受体通道峰电流升高（P〈0．01）；与R时比较，R组T1-T6时NMDA受体通道峰电流升高（P〈0．01）；与T5时比较，R组T2-T4时、L时NMDA峰电流下降（P〈0．01）。结论瑞芬太尼可增强大鼠脊髓背角神经元NMDA受体功能，于洗脱后达峰效应，芬太尼无此作用。Objective To investigate the effect of remifentanil and fentanyl on N-methyl-D-aspartate （NMDA） receptor currents in rat spinal cord dorsal horn neurons. Methods Whole-cell patch-clamp technique was used to record the NMDA receptor currents. The primary cultured E14SD rat spinal cord dorsal horn neurons （DH cells） were randomly divided into 3 groups （ n = 10 each） ： remifentanil group （group R）, fentanyl group （group F） and control group （group C）. DH cells were perfused with 4 nmol/L remifentanil （group R） or 10μmol/L fentanyl （group F） for 60 min followed by washout. NMDA receptor currents were recorded immediately after administration （To）, at 15, 30, 45 and 60 min of action of drugs （T1-4）, and at 15 and 30 min （T5-6 ） after washout. Results Compared with group C, no significant change in the peak NMDA receptor current was found at each time point in group F and at To and T1 in group R （ P 〉 0.05）, and the peak NMDA receptor current was significantly increased at T2-6 in group R （ P 〈 0.01 ）. The peak NMDA receptor current was significantly higher at T2-6 than at To, while lower at T2-4 and T6 than at T5 in group R （ P 〈 0.01 ）. Conclusion Remifentanil can in- crease NMDA receptor function in rat spinal cord horn neurons, and the peak effect is reached after washout, but fentanyl dose not have the effect.

关 键 词：哌啶类 受体 N-甲基-D-天冬氨酸 药物耐受性 

分 类 号：R614[医药卫生—麻醉学]