异丙酚联合白藜芦醇预先给药对大鼠肝脏缺血再灌注损伤的影响  

Effects of propofol combined with resveratrol pretreatment on hepatic ischemia-reperfusion injury in rats

作　　者：申新[1] 赵鸽[1] 刘婷婷[1] 王瑞[1] 刘琳[1] 

机构地区：[1]西安交通大学医学院第一附属医院麻醉科,710061

出　　处：《中华麻醉学杂志》2013年第3期371-375,共5页Chinese Journal of Anesthesiology

摘　　要：目的评价异丙酚联合白藜芦醇预先给药对大鼠肝脏缺血再灌注损伤的影响。方法健康成年雄性sD大鼠108只，体重220～270g，6～7周龄，采用随机数字表法，将其分为6组（n=18）：假手术组（S组）、缺血再灌注组（I／R组）、溶剂组（TW-80组）、异丙酚组（P组）、白藜芦醇组（R组）和异丙酚＋白藜芦醇组（P＋R组）。采用阻断第一肝门30min后恢复灌注的方法制备大鼠肝脏缺血再灌注模型。于缺血前10rain经股静脉给药，P组静脉输注异丙酚10mg·kg^-1·h^-1至术毕；R组静脉注射白藜芦醇10mg/kg；P＋R组静脉注射白藜芦醇10mg／kg后输注异丙酚10mg·kg^-1·h^-1至术毕；S组和I／R组以等容量生理盐水替代，TW-80组静脉输注等容量TW-80。于再灌注3、6、12h时经上下腔静脉取血样2ml，测定血清ALT、AST活性，取肝组织，测定SOD、髓过氧化物酶（MPO）活性、诱导型一氧化氮合酶（iNos）表达、MDA和一氧化氮（NO）含量，观察肝组织病理学改变。结果与S组比较，其余各组血清ALT、AST活性、肝组织MDA、NO含量、MPO活性和iNOS表达水平升高，SOD活性降低（P〈0．05）。与I／R组比较，P组、R组及P＋R组血清ALT、AST活性、肝组织MDA、NO含量、MPO活性和iNOS表达水平降低，SOD活性升高（P〈0．05），TW-80组上述各指标差异无统计学意义（P〉0．05）。与P组和R组比较，P＋R组肝组织SOD活性升高，其余指标均明显降低（P〈0．05）。P组、R组及P＋R组肝组织病理学损伤程度轻于I／R组。结论异丙酚联合白藜芦醇预先给药可减轻大鼠肝脏缺血再灌注损伤，其效果优于两者单独应用；机制与提高机体抗氧化能力，抑制脂质过氧化反应，减少内源性NO生成有关。Objective To evaluate the effects of propofol combined with resveratrol on hepatic ischemia- reperfusion （I/R） injury in rats. Methods One hundred and eight male Sprague-Dawley rats, aged 6-7 weeks, weighing 220-270 g, were randomly divided into 6 groups （ n = 18 each） ： sham operation group （group S） ; I/R group; solvent group （group TW-80） ; propofol group （group P） ; resveratrol group （group R） ; propofol combined with resveratrol group （group P ＋ R） . The animals were anesthetized with intraperitoneal 10% chloral hydrate 3.5- 4.0 ml/kg. Liver ischemia was produced by clamping the first hepatic portal for 30 min, followed by 12 h reperfu- sion. In group P, propofol was infused intravenously at 10 mg·kg-1 . h-~ starting from 10 min before ischemia until the end of operation. In group R, resveratrol 10 mg/kg was injected intravenously at 10 min before ischemia. In group P＋ R, resveratrol 10 mg/kg was injected intravenously and then propofol was infused intravenously at 10 mg· kg- 1 . h- 1starting from 10 min before ischemia until the end of operation. The equal volume of normal saline was given instead in groups S and I/R, and the equal volume of TW-80 was given instead in group TW-80. Six rats in each group were chosen at 3, 6 and 12 h of reperfusion and blood samples were taken from the superior and inferior vena cava for measurement of serum alanine aminotransferase （ALT） and aspartate amino transferase （AST） activities. The rats were then sacrificed and livers were removed for microscopic examination and for determination of superoxide dismutase （SOD） and myeloperoxidase （MPO） activities, inducible nitric oxide synthase （iNOS） ex- pression, and malondialdehyde （MDA） and nitric oxide （NO） contents in liver tissues. Results Compared with group S, the serum ALT and AST activities, and MDA and NO contents, MPO activity and iNOS expression in liv- er tissues were significantly increased, and the activities of SOD were decreased in the other four grou

关 键 词：二异丙酚 藜芦生物碱类 再灌注损伤 肝 

分 类 号：R6[医药卫生—外科学]