苦柏妇炎栓精制工艺优选  

Optimization of Purification Technology of Kubai Fuyan Suppositories

作　　者：姚琳[1] 高菲[1] 孙妍[1] 王伟明[1] 

机构地区：[1]黑龙江省中医研究院,哈尔滨150036

出　　处：《中国实验方剂学杂志》2013年第11期38-41,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:优选苦柏妇炎栓的精制工艺。方法:运用大孔吸附树脂法,以盐酸小檗碱、苦参碱、蛇床子素含量为指标,通过单因素试验筛选树脂型号及其精制工艺;采用HPLC测定指标成分含量。结果:采用HPD-400A型大孔吸附树脂,其最佳精制工艺为树脂(干重)-药材质量(1∶3),上样液质量浓度1.0 g.mL-1,上样液pH 8,洗脱流速1.5 BV.h-1,加5 BV水洗脱,之后分别用5 BV的20%乙醇和40%乙醇洗脱,合并乙醇洗脱液,减压回收,干燥,即得精制浸膏。结论:经HPD-400A型大孔吸附树脂精制后,栓剂载药量提高了4.3倍;优选的精制工艺稳定可行,可用于苦柏妇炎栓的工业化生产。Objective： To optimize purification technology of Kubai Fuyan suppositories. Method： Macroporous resin method was adopted,with the content of berberine hydrochloride,matrine and osthole as index,type of macroporous resin and its purification technology was screened by single factor test.The content of indicators ingredients were determined by HPLC. Result： HPD-400A type macroporous resin was selected with optimum purification technology was as following：ratio of resin and crude drug 1： 3,the sample solution concentration of g·mL^-1 with pH 8,elution flow rate of 1.5 BV·h^-1,eluated with 5 BV water,then eluated with 5 BV of 20% ethanol and 40% ethanol,merged ethanol eluent,recovered,dried,then received purified extracts. Conclusion： Drug loading of suppositories increased 4.3 times after purified with HPD-400A type macroporous resin.This optimized purification technology was stable and feasible,which could be used for industrial production of Kubai Fuyan suppositories.

关 键 词：大孔吸附树脂 盐酸小檗碱 苦参碱 蛇床子素 

分 类 号：R283.6[医药卫生—中药学]