HPLC测定复方豆蔻合剂中橙皮苷含量  

Determination of Hesperidin in Fufang Doukou Mixtures by HPLC

作　　者：赵晓红[1,2] 杨莹[2] 申睿[2] 雷虹[2] 慕磊[2] 郭琪[2] 

机构地区：[1]河北北方学院,河北张家口075000 [2]北京军区联勤部药品仪器检验所,北京100071

出　　处：《中国实验方剂学杂志》2013年第11期61-63,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：全军军队中医药科研专项课题(10ZYZ204)

摘　　要：目的:建立HPLC测定复方豆蔻合剂中橙皮苷含量的方法。方法:色谱条件为Agilent Zorbax SB-C18色谱柱(4.6mm×250 mm,5μm),流动相甲醇-2%乙酸溶液(35∶65),流速1.0 mL.min-1,检测波长283 nm,柱温35℃,进样量10μL。结果:橙皮苷在2.084～83.36 mg.L-1与其峰面积呈良好线性关系(r=0.999 9),平均回收率98.6%,RSD 1.8%。结论:该方法简便准确、灵敏度高、重复性好,为复方豆蔻合剂的质量控制提供参考。Objective： To develop a HPLC method for determining hesperidin in Fufang Doukou mixtures. Method： Chromatography conditions were as followings：Agilent Zorbax SB-C18 column （4.6 mm×250 mm,5 μm）, mobile phase of methanol-2% acetic acid （35： 65）,a flow rate of 1.0 mL·min-1,the detection wavelength at 283 nm,the column temperature at 35 ℃,the injection volume 10 μL. Result： A good linear relationship between peak areas and the concentration of hesperidin was observed within the range of 2.084-83.36 mg·L^-1r=0.999 9）,the average recovery was 98.6% with RSD 1.8%. Conclusion： This method was rapid,accurate and reliable,it could provide a reference for quality control of Fufang Doukou mixtures.

关 键 词：复方豆蔻合剂 橙皮苷 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]