藏药镰形棘豆的化学成分及其抗肿瘤活性研究  

Study on Chemical Constituents and Antitumor Activity of Oxytropis falcata

作　　者：顾青[1] 蔡银娜 杨光明[1,2] 蔡宝昌[1,2] 潘扬 

机构地区：[1]南京中医药大学江苏省中药炮制重点实验室,南京210023 [2]国家中医药管理局中药炮制标准重点实验室,南京210023

出　　处：《中国实验方剂学杂志》2013年第11期72-75,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30902012);江苏省中医药局项目(LB09026)

摘　　要：目的:研究藏药镰形棘豆Oxytropis falcata的化学组成及其抗肿瘤活性。方法:通过硅胶柱色谱、制备TLC分离纯化镰形棘豆的化学成分,通过NMR等波谱技术解析鉴定化合物结构,通过MTT法研究化合物1,3,5抑制人肝癌细胞HepG2、小鼠黑色素瘤细胞B16F10、人肝癌细胞SMMC-7721、人肝癌细胞HuH7以及人乳腺癌细胞MDA-MB-231的增殖影响。结果:从镰形棘豆乙醇提取物中分离得到了5个化合物,分别为7-甲氧基二氢黄酮(7-methoxy flavanone,1),7-羟基二氢黄酮(7-hydroxy flavonone,2),2',4'-二羟基査耳酮(2',4'-dihydroxy chalcone,3),2',4'-二羟基-4'-甲氧基查尔酮(2',4'-dihydroxy-4'-methoxy chalcone,4),N-(2-苯乙基)肉桂酰胺(N-2-phenylethyl cinnamamide,5)。四甲基偶氮唑盐比色(MTT)结果表明化合物3具有较好的抗肿瘤活性,其IC50分别为68.60,169.67,105.88,175.18,172.37μmol.L-1。结论:其中化合物5为首次从该种属中分离得到,化合物3对5种肿瘤细胞的增殖具有明显的抑制作用。Objective： To study the constituents from Oxytropis falcate and their antitumor activity. Method： Constituent isolation and purification was carried by column chromatography and their structures were elucidated on the basis of spectral data analysis.Cytotoxicity of compounds 1, 3, 5 was assessed using MTT colorimetric method against HepG2, B16F10, SMMC-7721, HuH7 and MDA-MB-231 tumor cell lines. Result： Five compounds（1-5） were elucidated as 7-methoxy flavanone （1）, 7-hydroxy flavonone （2）, 2＇, 4＇-dihydroxy chalcone （3）, 2＇, 4＇-dihydroxy-4＇-methoxy chalcone （4） and N-2-phenylethylcinnamamide （5）. The compounds 3 showed the strongest cytotoxic effect on these five cell lines with IC50 values of 68.60, 169.67, 105.88, 175.18, 172.37 μmol·L^-1respectively. Conclusion： Compound 5 was isolated from this genus for the first time. Compound 3 showed significant antitumor activity.

关 键 词：镰形棘豆 化学成分 抗肿瘤活性 

分 类 号：R284.1[医药卫生—中药学]