HPLC-MS^n法测定加拿大原产地西洋参不同入药部位的人参皂苷含量  

Research on Concentration of Ginsenoside in Different Medical Parts of Panax quinquefolium by HPLC-MS^n

作　　者：黄艳菲[1] 刘永恒[1] 李艳丹[1] 丁玲[1] 左旭[1] 刘圆[1] 

机构地区：[1]西南民族大学民族医药研究院,成都610041

出　　处：《中国实验方剂学杂志》2013年第11期86-91,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家外国专家局项目(2012);国家科技部支撑计划项目(2012BAI27B07);中央高校基本科研业务费专项资金项目优秀科研团队及重大孵化项目(11NZYTH02)

摘　　要：目的:采用HPLC-MSn法对加拿大原产地采收的西洋参根、茎和叶中的10种人参皂苷的含量进行测定。方法:采用HPLC-MSn法,色谱柱为DIKMA diamonsil(4.6 mm×250 mm,5μm),流动相为乙腈-0.05%磷酸水和乙腈-0.05%乙酸水,流速0.3 mL.min-1,柱温35℃,检测波长203 nm。质谱条件:正离子检测模式,鞘气10 L.min-1,辅助气10 L.min-1,喷雾电压4.5 kV,毛细管温度320℃,毛细管电压30 V。结果:10种人参皂苷的含量在一定范围内线性关系良好(r>0.999),平均回收率为95%～102%,RSD<2.68%。西洋参不同入药部位单体人参皂苷含量差异较大,西洋参叶的总皂苷与西洋参根的总皂苷相当,西洋参叶样品S1的总皂苷甚至高于西洋参根的总皂苷;西洋参茎的总皂苷含量则远低于叶和根中的含量;单体人参皂苷则以Rb2、Rb3在西洋参叶中有很高的含量,Re,Rb1在根中有很高的含量。结论:该方法简便、准确、重复性好,可用于西洋参的定量定性检测;建议西洋参叶可以作为人参皂苷的新来源。Objective： High-performance liquid chromatography array mass spectrometry （HPLC-MS） method was developed for determination of the concentration of 10 different ginsenosides of different medical parts of Panax quinquefolium, which collected from original habitat in Ontario of Canada. Method： Sample solution was separated on a DIKMA diamonsil （4.6 mm×250 mm, 5 μm）. Acetonitrile-0.05% phosphoric acid aqueous solution and acetonitrile-0.05% acetic acid aqueous solution were used as mobile phase and the flow rate through the HPLC column was 0.3 mL·min^-1and the entire effluent was directed to the mass spectrometer. The column temperature was kept at 35 ℃ and the UV detection wavelength was set at 203 nm. MS analysis was monitored in positive mode. The conditions of ESI source were as follows： sheath gas flow rate, 10 L·min^-1; sweep gas flow rate, 10 L·min^-1; spray voltage, 4.5 kV; capillary temperature, 320 ℃; capillary voltage 30 V. Result： All calibration curves showed good linearity （r〉0.999） within the test ranges. The average recovery of the method was between 95% and 102%, RSD〈2.68%. The concentration of single ginsenoside of root, stem and leaf was quite different; that of Re and Rb1 of root and that of Rb2 and Rb3 of leaf was higher than others; that of stem was lower than that of root and leaf, moreover that of some samples of leaf was higher than that of root. Conclusion： The method is simple, accurate, replicate and suitable for the determination of P. quinquefolium. It is suggested that the leaf of P. quinquefolium can be used a new resource of ginsenoside.

关 键 词：西洋参 根 茎 叶 人参皂苷 HPLC—MS 

分 类 号：R284.1[医药卫生—中药学]