蟒蛇油GC指纹图谱研究  

GC Fingerprint of Python Oil

作　　者：张仲敏[1] 黄国鑫[1] 易宇阳[1] 石书江[1] 冯学轩[1] 苏子仁[1] 曾惠芳[2] 

机构地区：[1]广州中医药大学,广州510006 [2]广州中医药大学第一附属医院,广州510006

出　　处：《中国实验方剂学杂志》2013年第11期118-122,共5页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究蟒蛇油GC指纹图谱,为蟒蛇油的行业质量控制提供依据。方法:采用气相色谱法,以油酸甲酯为内参照物,采用石英毛细管柱007-225(0.25μm×30 m×0.25 mm),进样口温度280℃,检测器温度280℃,载气流速0.8 mL.min-1,分流比20∶1,进样量为1μL。对24批蟒蛇油进行研究,采用药典委员会颁布的中药色谱指纹图谱相似度评价系统2004A对指纹图谱进行了分析;采用中药色谱指纹图谱相似度评价系统2004B对蟒蛇油与棕榈油、花生油、猪油、普通蛇油的指纹图谱进行比较。结果:24批蟒蛇油样品有9个共有峰,与蟒蛇油GC对照指纹图谱的相似度对比均>0.998,24批蟒蛇油样品的参照峰的丰度范围在0.454～0.493。并采用相似度及参照峰丰度区分了蟒蛇油与棕榈油、花生油、猪油、普通蛇油等油脂。结论:该方法简便、精密性,重复性,稳定性良好,可作为蟒蛇油的质量评价方法。Objective： To study the fingerprints of python oil by GC and establish the method of standard quality for python oil. Method： Methyl oleate was used as the internal standard substance. A Quartz capillary column was used （0.25 μm×30 m×0.25 mm）. Injector temperature was set at 280 ℃. FID temperature was 280 ℃. The carrier gas flow rate is 0.8 mL·min^-1. Distributary ratio was 20： 1.Incoming sample amount was 1 μL. The obtained data was analyzed by computer aided similarity evaluation and cluster analysis software. Result： There were 9 common peaks in 24 groups of python oil. The similarity degree between 24 python oil groups and standard python oil was found to be higher than 0.998.The range of abundance of reference peaks in 24 python oil groups was 0.454-0.493.The similarity and reference to the peak abundance distinguish the Python oil and other oil such as palm oil, peanut oil, lard oil and average snake oil. Conclusion： The method is simple, accurate, repeatable and reliable, which can be used as a quality control method for Python oil.

关 键 词：蟒蛇油 气相色谱 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]