基于色谱指纹图谱的苍术挥发油多成分体内药代动力学研究  

Studies on Pharmacokinetics of Multi-constituents of Atractylis Oil based on Chromatographic Fingerprint

作　　者：陶野[1,2] 张贝贝[2] 付梅红[2] 许海玉[2] 方婧[2] 王维皓[2] 李德凤[2] 张毅[2] 杨洪军[2] 

机构地区：[1]天津中医药大学,天津300193 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2013年第11期156-159,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国中医科学院基本科研业务费自主选题课题(Z02063);国家科技支撑计划项目(2008BAI51B03)

摘　　要：目的:建立了茅苍术挥发油中7个成分的体内分析方法。方法:采用内标法测定了给药后不同时间点7个化合物的血药浓度,并通过DAS 3.0软件计算出药代动力学参数,以自定义各成分的权重系数进行茅苍术挥发油整合药代动力学研究,分别获得化合物1～7及其整合的血浆浓度-时间曲线,化合物2～7和整合的药动学参数。结果:整合药动学参数T1/2,Tmax,Cmax,AUC(0-∞)和MRT(0-∞)分别为8.01,4.00 h,3.84 mg.L-1,42.31 mg.L-1.h-1,11.65 h。结论:研究思路符合中医药作用特点,对多组分苍术挥发油的药代动力学做了一些初步的探索。Objective： To investigate pharmacokinetics process of the major components of Atractylodes lancea oil in rats. Method： A preliminary HPLC method based on internal standard method was employed to analysis 7 constituents simultaneously in plasma and to study their pharmacokinetics after oral administration of volatile oil from A. lancea in rats. Pharmacokinetic parameters of compound 2-7 were acquired by DAS 3.0 software and the integrative concentration-time curve was obtained based on the corresponding weighting coefficients. Result： Integrative pharmacokinetic parameters was as follows： T1/2 was 8.01 h,Tmax was 4.00 h,Cmax was 3.84 mg·L^-1,AUC（0-∞） was 42.31 mg·L^-1·h^-1 and MRT（0-∞） was 11.65 h, respectively. Conclusion： This study was thought as an appropriate method for traditional Chinese medicine and we carried out some exploratory survey for pharmacokinetics of volatile oil from A. lancea.

关 键 词：茅苍术挥发油 内标法 整合药代动力学 

分 类 号：R285.5[医药卫生—中药学] R284.1[医药卫生—中医学]