二苯乙烯苷对Aβ_(25-35)致神经干细胞损伤的保护作用  

Protective Effect of Tetrahydroxy Stilbene Glycoside on the NSCs Injury Induced by Aβ_(25-35)

作　　者：韩文文[1,2] 张玉莲[1] 周震[1] 黄建华[3] 张琳琳[1] 

机构地区：[1]天津中医药大学第二附属医院脑病科,天津300150 [2]天津中医药大学,天津300073 [3]复旦大学附属华山医院,上海200030

出　　处：《中国实验方剂学杂志》2013年第11期160-163,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展计划(973)课题(2010CB530405);国家自然科学基金项目(81102577H2708)

摘　　要：目的:观察不同浓度二苯乙烯苷(TSG)对β-淀粉样肽(Aβ25-35,25μmol.L-1)致损伤的神经干细胞(NSCs)生长、增殖及自我更新能力的影响,探讨TSG对Aβ25-35致NSCs损伤的保护作用。方法:从孕14 d昆明小鼠体外提取神经干细胞,以25μmol.L-1浓度Aβ25-35诱导NSCs建立稳定的AD细胞模型,与不同浓度二苯乙烯苷共同孵育,分为对照组、模型组(Aβ25-3525μmol.L-1)、TSG 1×10-8mol.L-1+Aβ25-35组、TSG 1×10-7mol.L-1+Aβ25-35组、TSG 1×10-6mol.L-1+Aβ25-35组,共5组,分别采用EDU化学荧光染色法检测NSCs增殖情况;采用WST-1细胞增殖及细胞毒性检测法检测不同时段NSCs的细胞活性;体外培养7 d后通过神经干细胞球计数法观察NSCs的自我更新能力。结果:与模型组相比,二苯乙烯苷各剂量组均能在一定程度上抑制Aβ25-35对NSCs的损伤,细胞生长状态良好,细胞增殖率、细胞活性及神经干细胞球计数与模型组相比均有显著性差异(P<0.01)。结论:二苯乙烯苷对Aβ25-35诱导的神经干细胞损伤具有一定保护作用。Objective： To observe the effects of different concentrations of tetrahydroxy stilbene glycoside （TSG） on the growth and proliferation of neural stem cells（NSCs） which were injured by Aβ25-35, to discuss the protective effects of TSG on the NSCs injury induced by Aβ25-35. Method： NSCs were extracted on both sides of brain cortex from the embryos of 14 days of Kunming mice pregnancy,NSCs were cultured by Aβ25-35（25 μmol·L^-1 to establish cell model of Alzheimer＇s disease （AD） and then were cultured with TSG. All cells were divided into 5 groups,control group; model group（Aβ25-35 25 μmol·L^-1, （TSG 1×10^-8mol·L^-1Aβ25-35）group, （TSG 1×10^-7 mol·L^-1Aβ25-35）group and （TSG 1×10^-6mol·L^-1Aβ25-35）group.Using the chemical fluorescence staining method （EDU） to detect the proliferation of NSCs; applicating WST-1 cell proliferation and cell toxicity test to detect the activity of NSCs of different time and then after cultured 7 days in vitro, counting neurospheres was used to observe the update ability of NSCs. Result： TSG could protect NSCs from Aβ25-35 damage. Compared with model group, the morphology, reproduction rate, cell viability and ball count of NSCs were significant different from TSG group （P〈0.05或P〈0.01）. Conclusion： TSG can protect the NSCs injury induced by Aβ25-35 in some ways.

关 键 词：补肾中药 二苯乙烯苷 神经干细胞 增殖 老年性痴呆 Β-淀粉样肽 

分 类 号：R285.5[医药卫生—中药学]