喘平方对哮喘豚鼠肺泡灌洗液中IgE,TGF-β_1及TNF-α的影响  

Effect of Chuanping Compound on IgE,Transforming Growth Factor-beta 1 and Tumor Necrosis Factor-alpha in BALF of Guinea Pig with Asthma

作　　者：张雄飞[1] 黄娟萍[1] 罗裕[1] 谢吉福[1] 柴金珍[1] 江力[1] 朱盛山[1] 

机构地区：[1]广东药学院中药开发研究所,广州510006

出　　处：《中国实验方剂学杂志》2013年第11期172-175,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"十二五"重大新药创制科技重大专项(2011ZX09102-011-01)

摘　　要：目的:探讨喘平方防治支气管哮喘的作用机制。方法:取白化豚鼠60只,随机分为正常组、模型组、麻黄组(0.20 g.kg-1)、洋金花组(0.07 g.kg-1)、喘平方组(0.32 g.kg-1)、地塞米松组(7.5×10-4mg.kg-1),通过对豚鼠ip卵蛋白致敏并雾化吸入激发,建立实验性哮喘豚鼠模型;治疗组自注射第14天起每天ig给药1次,连续7 d,正常组及模型组给予生理盐水。观察豚鼠的一般情况,豚鼠肺泡灌洗液中免疫球蛋白E(IgE),转化生长因子β1(TGF-β1),肿瘤坏死因子α(TNF-α)的变化,肺组织病理情况。结果:各组豚鼠肺泡灌洗液中IgE,TGF-β1,TNF-α分别为:正常组IgE(68.25±5.92)mg.L-1,TGF-β1(78.72±10.92)ng.L-1,TNF-α(388.02±55.61)ng.L-1;模型组IgE(137.28±14.38)mg.L-1,TGF-β1(172.39±14.04)ng.L-1,TNF-α(752.76±51.25)ng.L-1;喘平方组IgE(76.35±6.22)mg.L-1,TGF-β1(115.76±9.17)ng.L-1,TNF-α(569.32±39.7)ng.L-1;哮喘组及喘平方组豚鼠肺泡灌洗液中IgE,TGF-β1,TNF-α含量均高于正常组(P<0.05);喘平方组豚鼠肺泡灌洗液中IgE,TGF-β1,TNF-α含量均低于哮喘组(P<0.05)。结论:喘平方能够通过下调豚鼠肺泡灌洗液中IgE,TGF-β1,TNF-α含量,可降低气道高反应性、减轻气道炎症症状、控制或延缓气道纤维化进程。Objective： To investigate the therapeutic effect of Chuanping compound on guinea pigs with asthma. Method： All guinea pigs were given intraperitoneal injection of ovalbumin（OVA）and forced inhalation of atomized OVA to induce asthma. From the 14th day of modeling,the treatment groups were given the corresponding drugs according to the experimental design,ephedra groups （0.20 g·kg^-1）, datura flower group （0.07 g·kg^-1）, Chuanping compound group （0.32 g·kg^-1）, dexamethasone group （7.5×10^-4 mg·kg^-1）, and the normal group and model group were given normal saline（NS）,once a day for 7 days. The general state of the guinea pigs and the changes of immunoglobulin E（IgE）;transforming growth factor-beta 1 （TGF-β1） and tumor necrosis factor-alpha （TNF-α） in bronchoalveolar lavage fluid （BALF）were observed. Result： The levels of IgE,TGF-β1 and TNF-α in BALF of guinea pigs： normal group IgE（68.25±5.92） mg·L^-1TGF-β1（78.72±10.92） ng·L^-1 TNF-α（388.02±55.61） ng·L^-1; model group IgE（137.28±14.38） mg·L^-1TGF-β1（172.39±14.04） ng·L^-1TNF-α（752.76±51.25） ng·L^-1Chuanping compound group IgE（76.35±6.22） mg·L^-1TGF-β1（115.76±9.17） ng·L^-1TNF-α（569.32±39.7） ng·L^-1The levels of IgE,TGF-β1 and TNF-α in BALF of the model group and Chuanping compound group were higher than those in the normal group. After treatment,IgE,TGF-β1 and TNF-α levels in BALF of chuanping group were lower than those in the model group. Conclusion： Chuanping compound has an effect of reducing airway hyperresponsiveness,alleviating airway inflammation,controlling or delaying the process of airway fibrosis through up-regulation of IgE,TGF-β1 and TNF-α levels in BALF of guinea pigs.

关 键 词：哮喘 作用机制 IGE 转化生长因子Β1 肿瘤坏死因子Α 喘平方 

分 类 号：R285.5[医药卫生—中药学]