灯盏生脉胶囊对博莱霉素诱导的小鼠肺纤维化的干预作用  

Protective Effects of Dengzhan Shengmai Capsules against Bleomycin-induced Pulmonary Fibrosis in C57BL/6 Mice

作　　者：曾嫱[1] 谭亚夏[1] 孙红[1] 肖丹[1] 刘蓉[1] 朱琦[1] 杨通[1] 钟南山[1] 

机构地区：[1]广州医学院第一附属医院呼吸疾病国家重点实验室,广州510120

出　　处：《中国实验方剂学杂志》2013年第11期199-203,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广州呼吸疾病国家重点实验室开放课题项目(2007DA780154F0908)

摘　　要：目的:研究灯盏生脉胶囊对博莱霉素诱导的小鼠肺纤维化的干预作用。方法:将40只C57BL/6雌性小鼠随机分为盐水对照组(NS)、模型组(Model)、灯盏生脉组(DZSM)和N-乙酰半胱氨酸阳性对照组(N-AC),NS组气管滴注0.1 mL生理盐水,其余3组按5 mg.kg-1剂量滴注博莱霉素溶液复制肺纤维化模型,以后每天分别给予生理盐水、灯盏生脉溶液(222mg.kg-1.d-1)、N-乙酰半胱氨酸溶液(489.6 mg.kg-1)ig治疗,连续给药21 d。实验过程中隔天记录小鼠的生存状态及体重变化,在完成给药后观察肺顺应性和肺阻力的改变状况。留取肺泡灌洗液检测细胞转化生长因子-β(TGF-β)、肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)含量,观察博莱霉素引起的肺炎症改变;留取肺组织,测定肺组织中羟脯氨酸含量,并分别行HE染色和Masson's染色,观察肺组织的炎症变化和纤维化程度。结果:给予博莱霉素后,各组小鼠体重显著下降,进食减少,肺阻力升高(P<0.05),肺泡灌洗液中TGF-β,TNF-α,IL-1β含量显著升高(P<0.05);灯盏生脉胶囊干预组小鼠体重下降减缓,肺阻力显著下降(P<0.05),肺泡灌洗液中TGF-β,TNF-α,IL-1β含量明显降低(P<0.05);肺组织的病理切片显示肺部炎症及肺纤维化程度均有不同程度的减轻。结论:灯盏生脉胶囊能改善博莱霉素诱导的肺纤维化小鼠的虚弱状态,增强小鼠肺顺应性,降低肺阻力,抑制小鼠的肺组织炎症反应,减轻肺纤维化程度。Objective： To evaluate the protective effects of Dengzhan Shengmai capsules（DZSM） on bleomycin-induced pulmonary fibrosis, and to discover new functions and applications for DZSM capsules. Method： All 40 C57BL/6 female mice were divided into four groups averagely and randomly： NS group, model group, DZSM group and N-acetyl-N- cysteine（N-AC） group. Pulmonary fibrosis mouse models were generated by intratracheally instilled of bleomycin solution and then treated with saline, N-AC solution and DZSM solution for 21 days respectively. The body weight and life state of each mouse was recorded every two days. After the last treatment, the lung compliance and resistance were tested using Buxco invasive lung mechanics machine. Lung tissues were collected after the mice were killed and observed following staining with HE solution or Masson＇s solution to estimate the inflammatory level and fibrosis degree respectively. bronchoalveolar lavage fluid（BALF） were collected for inflammatory cytokines assay. Hydroxyproline levels were also detected to estimate fibrosis degree. Result： The body weights and appetites were significantly decreased after giving bleomycin. DZSM solution （222 mg·kg^-1·d^-1） could retard the loss of body weights, slightly reduce the lung compliance and improve the lung resistance significantly （P〈0.05）. The inflammatory cytokines such as transforming growth factor-β （TGF-β）,tumor necrosis factor-α （TNF-α）,interleukin-1β （IL-1β） in BALF and the hydroxyproline level in lung tissue were also significantly down-regulated （P 〈0.05）. Furthermore, the inflammatory state and fibrosis level were also relieved in pathological specimens. Conclusion： DZSM could improve the weakness in bleomycin-induced pulmonary fibrosis mouse models, ameliorate the lung compliance, lower the lung resistance, inhibit the inflammatory statement, decrease inflammatory cytokines levels and relieve fibrosis.

关 键 词：肺纤维化 灯盏生脉胶囊 博莱霉素 炎症因子 

分 类 号：R285.5[医药卫生—中药学]