转化生长因子β_1刺激大鼠肺成纤维细胞增殖的体外研究  

Transforming Growth Factor-β_1 Stimulates the Proliferation of Rat Lung Fibroblasts in vitro

作　　者：侯雅静 高永红[2] 孙逸坤[2] 黄娟[3] 于会勇[3] 黄文通[3] 冯淬灵[3] 

机构地区：[1]山西省中西医结合医院呼吸科,太原030013 [2]北京中医药大学东直门医院重点实验室,北京100700 [3]北京中医药大学东直门医院呼吸科,北京100700

出　　处：《中国实验方剂学杂志》2013年第11期204-208,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81072769)

摘　　要：目的:探讨转化生长因子β1(TGF-β1)对大鼠肺成纤维细胞体外增殖的影响。方法:体外原代及传代培养SD大鼠肺成纤维细胞,传至第4代,进行细胞增殖实验。细胞随机分为5组,即无血清细胞基础培养液(DMEM)组、分别含10.0,5.0,2.5,1.25μg.L-1TGF-β1DMEM组。分别在24,48,72 h 3个不同刺激时间点应用四甲基偶氮唑盐比色法(MTT法)观察SD大鼠肺成纤维细胞增殖情况。进行统计学方差分析及多重比较,探讨TGF-β1刺激大鼠肺成纤维细胞增殖的最佳浓度和最佳时间点。结果:24 h时间点各组数据经方差分析,差异无统计学意义。48 h时间点各组数据结果进行非参数检验,P<0.05,差异有统计学意义。72 h时间点各组数据结果进行方差分析,P<0.05,差异有统计学意义;进一步进行多重比较:与空白组比较,TGF-β110.0,2.5μg.L-1组(P<0.05),TGF-β11.25,2.5μg.L-1组(P<0.01);其余各组比较均无显著差异。结论:TGF-β1刺激质量浓度2.5μg.L-1,作用于大鼠肺成纤维细胞72 h,细胞增殖显著。Objective： To explore stimulates the proliferation of transforming growth factor-β1 （TGF-β1） in vitro rat lung fibroblasts. Method： The rat lung fibroblast were cultured in vitro,the 4th generation of cells in logarithmic growth phase were used to proliferation experiments. These cells were randomly divided into five groups：serum-free Dulbecco＇s modified Eagle medium（DMEM） group, 10.0,5.0,2.5,1.25 μg·L^-1f TGF-β1 DMEM group. We selected the optimal stimulation point of TGF-β1 in three different stimulation time points of application（24,48,72 h） by the tetrazolium salt colorimetric method （MTT method） to explore the TGF-β1 stimulating rat lung fibroblast proliferation optimal concentration and the best time point by statistical analysis of variance and multiple comparison. Result： TGF-β1 in 24 h time point directly stimulated the 5 different groups of SD rat lung fibroblast proliferation based on MTT results by analysis of variance,the five groups were no significant difference. In 48 h time point,by the non-parametric test, P〈0.05, these groups were significantly different.Seventy-two h time point,by analysis of variance, P〈0.05, these groups were significantly different; further two multiple comparisons： control group,10.0, 2.5 μg·L^-1roup, P〈0.05, 2.5, 1.25 μg·L^-1re P〈0.01, the rest of the group, P〉0.05. Conclusion： 48 h, 72 h time point were observed to promote the proliferation of TGF-β1, and the best proliferative activity of stimulate concentration and time point of TGF-β1 were 2.5 μg·L^-172 h.In this concentration and time point, rat lung fibroblast proliferated significantly.

关 键 词：转化生长因子Β1 肺成纤维细胞 增殖 体外 

分 类 号：R285.5[医药卫生—中药学]